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Recombinant Bovine Protein Wnt (WNT5B), N-His

Cat. No.AP9C314

Product TypeAnimal Proteins

Size

Product Overview

BioVenic's Recombinant Bovine Protein Wnt (WNT5B), N-His is a recombinant protein expressed from E.coli. Its predicted molecular weight is 40.6 kDa. The purity is>95% (SDS-PAGE).

Specifications

Type Recombinant Protein
Species Bovine
Expression System E.coli
Purity >95% (SDS-PAGE)
Endotoxin < 1 EU/µg
Predicted Molecular Weight 40.6 kDa
Molecular Weight 41 kDa
Physical State Lyophilized
Formulation The buffer before lyophilization is 20 mM Tris, 150 mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Target Information

Bovine wingless type MMTV integration site family, member 5B (WNT5B) is a protein that plays a significant role in various developmental processes and cellular activities in cattle. This protein is involved in cell migration, proliferation, and differentiation, and it operates through the non-canonical β-catenin-independent Wnt signaling pathway, often acting as an antagonist to the canonical Wnt signaling. WNT5B's unique expression patterns and functions distinguish it from other Wnt family members, even those with which it shares high amino acid identity, such as WNT5A. It contributes to the regulation of tissue regeneration, embryonic development, and cell fate determination, and its aberrant signaling has been implicated in various diseases, including osteoarthritis, osteoporosis, obesity, type 2 diabetes mellitus, neuropathology, and certain cancers.

Protein Bovine Protein Wnt (WNT5B)
UniProt ID A0AAA9T3N5

Shipping and Storage

This product is shipped with dry ice. It is recommended to aliquot as needed and store at -80°C upon receipt. Reconstituted protein solution can be stored at 4°C for 1 week, at < -80°C for 12 months. Avoid repeated freezing and thawing.

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

User Note

  1. Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Aliquot the reconstituted solution to minimise freeze-thaw cycles.
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