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Recombinant Bovine Aspartate Aminotransferase (AST), N-His
Product Overview
BioVenic's Recombinant Bovine Aspartate Aminotransferase (AST), N-His is a recombinant protein expressed from E.coli expression system. Its predicted molecular weight is 50.0 kDa. The purity is greater than 95% (SDS-PAGE). The endotoxin level is <1 EU/μg (LAL). It can be used as a positive control or as an immunogen.
Specifications
Target Information
Glutamic-oxaloacetic transaminase 1 (GOT1), also known as aspartate aminotransferase, cytoplasmic, is encoded by the GOT1 gene in cattle. GOT1 is a pyridoxal phosphate-dependent enzyme that exists in both cytoplasmic and mitochondrial forms. It plays a role in amino acid metabolism, as well as the urea and tricarboxylic acid cycles. The enzyme catalyzes the reversible transfer of an amino group between aspartate and α-ketoglutarate to form oxaloacetate and glutamate. It is a gene implicated in protection from cellular stress.
Shipping and Storage
This product is shipped with dry ice. Avoid repeated freezing and thawing. Store for 4 weeks at 2-8°C, for 12 months at -80°C.
Documents
The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.
User Note
- Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute in 10 mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Aliquote the reconstituted solution to minimise freeze-thaw cycles.
References
- Dikmen, S. et al. Genome-Wide Association Mapping for Identification of Quantitative Trait Loci for Rectal Temperature during Heat Stress in Holstein Cattle. PloS one. 2013, 8: e69202.