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Recombinant Canine Leptin (LEP), N-His

Name: Recombinant Canine Leptin (LEP), N-His
SKU: AP7F384
Rating: 5 (1 reviews)

Cat. No.AP7F384

Product TypeAnimal Proteins

Size

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Product Overview

BioVenic's Recombinant Canine Leptin (LEP), N-His is a recombinant protein expressed from E.coli expression system. Its predicted molecular weight is 19.8 kDa. The purity is>90% (SDS-PAGE). The endotoxin level is <1 EU/μg (LAL). It can be used as a positive control or as an immunogen.

Specifications

Type Recombinant Protein

Species Canine

Expression System E.coli

Purity >90% (SDS-PAGE)

Endotoxin <1 EU/μg (LAL)

Predicted Molecular Weight 19.8 kDa

Molecular Weight 20 kDa

Physical State Lyophilized

Formulation 20 mM Tris, 150 mM NaCl Buffer(pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300)

Target Information

Leptin is encoded by the LEP gene in dogs. It represents a key molecule in obesity, able to modulate immune responses and foster chronic inflammatory response in peripheral tissues.

Protein Canine Leptin (LEP)

Protein Synonym leptin, OB, leptin (obesity homolog, mouse), obesity factor

Gene ID 403616

UniProt ID O02720

Shipping and Storage

This product is shipped with dry ice. Avoid repeated freezing and thawing. Store for 4 weeks at 2-8°C, for 12 months at -80°C.

Documents

Datasheet

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

User Note

Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute in 20 mM Tris, 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. Aliquote the reconstituted solution to minimise freeze-thaw cycles.

References

Cortese, L. et al. Leptin and immunological profile in obesity and its associated diseases in dogs. International journal of molecular sciences. 2019, 20: 2392.