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Recombinant Porcine Visfatin (VF), N-His
Product Overview
BioVenic's Recombinant Porcine Visfatin (VF), N-His is a recombinant protein expressed from E.coli expression system. Its predicted molecular weight is 59.1 kDa. The purity is>80% (SDS-PAGE). The endotoxin level is <1 EU/μg (LAL). It can be used as a positive control or as an immunogen.
Specifications
Target Information
Nicotinamide phosphoribosyltransferase (NAMPT), also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin (for its extracellular form), is an enzyme encoded by the NAMPT gene in pigs. NAMPT catalyzes the transfer of a phosphoribosyl group from 5-phosphoribosyl-1-pyrophosphate (PRPP) to nicotinamide, forming nicotinamide mononucleotide (NMN). NMN is a key intermediate in the NAD pathway, which is essential for various cellular processes. It is the rate-limiting enzyme in the biosynthesis of nicotinamide adenine dinucleotide.
Shipping and Storage
This product is shipped with dry ice. Avoid repeated freezing and thawing. Store for 4 weeks at 2-8°C, for 12 months at -80°C.
Documents
The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.
User Note
- Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute in 10 mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Aliquote the reconstituted solution to minimise freeze-thaw cycles.
References
- Szymanska, K. et al. The Effect of Visfatin on the Functioning of the Porcine Pituitary Gland: An In Vitro Study. Cells. 2023, 12: 2835.