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Hamster Leptin (LEP) ELISA Kit-Sandwich

Cat. No.EK3F6

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Hamster Leptin (LEP) ELISA Kit-Sandwich is designed for the quantitative determination of Hamster LEP in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Hamster Leptin (LEP).
Target Species Hamster
Species Reactivity Hamster
Detection Range 7.85-500 pg/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Leptin, also referred to as the obesity protein, is a hormone primarily produced by adipocytes, the cells in adipose tissue. It functions by binding to and activating the long form of leptin receptors (LEPRb), which are extensively expressed in various brain regions, particularly the hypothalamus. Research indicates that effective leptin signaling to the brain is involved in reducing hyperphagia in hamsters that have been refed with a low-fat (LF) diet. Leptin also influences immune function and bone growth, with low leptin levels associated with increased bone mineral apposition rate in Siberian hamsters.

Target/Biomarker Hamster LEP
Target Synonym OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein; Leptin; LEP

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

  1. Zhao, Ying et al. "Leptin resistance was involved in susceptibility to overweight in the striped hamster re-fed with high fat diet." Scientific reports vol. 8,1 920. 17 Jan. 2018.; Kokolski, Marie et al. "Photoperiod-Induced Increases in Bone Mineral Apposition Rate in Siberian Hamsters and the Involvement of Seasonal Leptin Changes." Frontiers in endocrinology vol. 8 357. 19 Dec. 2017
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