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Mouse Anti-Swine CD25 Monoclonal Antibody

Cat. No.VD10H14

Product TypeAnimal-targeted Antibodies

Size

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Product Overview

O10H14 monoclonal antibody is specific for Swine CD25, also know as Swine IL2RA or TCGFR. It was purified by affinity chromatography on Protein A from tissue culture supernatant. It can be applied for Flow Cytometry (FC) and Immunohistochemical (IHC).

Specifications

Application FC; IHC

Clonality Monoclonal Antibody

Classification Primary Antibody

Clone O10H14

Host Mouse

Target Species Swine

Species Reactivity Swine

Specificity Swine CD25

Isotype IgG1

Immunogen Con A activated swine peripheral blood lymphocytes.

Purification Protein A Purified

Purity ≥ 90% (SDS-PAGE)

Concentration 0.05 mg/mL

Conjugation Unconjugated

Preservative and Stabilizer 0.09% Sodium azide (NaN₃) 1% Bovine Serum Albumin

Buffer Phosphate buffered saline

Physical State Liquid

Target Information

Cluster of differentiation 25 (CD25) or interleukin 2 receptor alpha (IL2RA), is an immunodeficiency disorder associated with mutations in the interleukin 2 receptor alpha (CD25) gene (IL2RA). CD25 plays an important role in T cell proliferation, activation induced cell death and the actions of both regulatory (Treg) and effector (Teff) T cells. Swine CD25 immunoprecipitates a protein of ~65-70 kDa from activated lymphocyte preparations.

Target Swine CD25

Target Synonym IL2RA; IDDM10; IL2R; TCGFR; p55; IMD41; interleukin 2 receptor subunit alpha

Gene ID 396814

UniProt ID O02733

Shipping and Storage

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt (up to 12 months).

Documents

Datasheet

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

User Note

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

References

Attreed, Sarah E., et al. "A highly effective African swine fever virus vaccine elicits a memory T cell response in vaccinated swine." Pathogens 11.12 (2022): 1438.