This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Recombinant Bovine Cholesterol Side-Chain Cleavage Enzyme, Mitochondrial (CYP11A1)
Product Overview
BioVenic's Recombinant Bovine Cholesterol Side-Chain Cleavage Enzyme, Mitochondrial (CYP11A1) is a recombinant protein expressed from E.coli. Its predicted molecular weight is 17.4 kDa. The purity is>95% (SDS-PAGE).
Specifications
Target Information
Bovine cholesterol side-chain cleavage enzyme, mitochondrial, also known as cytochrome P450scc (CYP11A1), is a crucial enzyme in the biosynthesis of steroid hormones. It is responsible for catalyzing the conversion of cholesterol to pregnenolone, which is the first and rate-limiting step in the production of all steroid hormones. This reaction involves initial hydroxylation at the C22 position, followed by a second hydroxylation at the C20 position, and finally, the oxidative cleavage of the C20-C22 bond, resulting in the formation of pregnenolone. CYP11A1 is a mitochondrial enzyme that requires electrons provided by NADPH through a short electron transport chain involving adrenodoxin reductase and adrenodoxin.
Shipping and Storage
This product is shipped with dry ice. It is recommended to aliquot as needed and store at -80°C upon receipt. Reconstituted protein solution can be stored at 4°C for 1 week, at < -80°C for 12 months. Avoid repeated freezing and thawing.
Documents
The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.
User Note
- Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Aliquot the reconstituted solution to minimise freeze-thaw cycles.