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Recombinant Equine Interleukin 18 (IL18), N-His
Product Overview
BioVenic's Recombinant Equine Interleukin 18 (IL18), N-His is a recombinant protein expressed from E.coli expression system. Its predicted molecular weight is 21.7 kDa. The purity is greater than 95% (SDS-PAGE). The endotoxin level is <1 EU/μg (LAL). It can be used as a positive control or as an immunogen.
Specifications
Target Information
Interleukin-18 (IL-18), previously known as interferon-gamma (IFN-γ) inducing factor (IGIF), is a potent pro-inflammatory cytokine. It is produced by a variety of cell types, including both hematopoietic cells and non-hematopoietic cells. While its initial recognition was in Kupffer cells (liver-resident macrophages), IL-18 is constitutively expressed in non-hematopoietic cells such as intestinal epithelial cells, keratinocytes, and endothelial cells. This cytokine plays multiple roles in both innate and acquired immunity. Elevated levels of IL-18 have been observed in the blood of patients with immune-related diseases like rheumatoid arthritis, systemic lupus erythematosus, type I diabetes mellitus, atopic dermatitis, psoriasis, and inflammatory bowel disease. Interleukin 18 is encoded by the IL18 gene in horses. It is a potent pro-inflammatory cytokine involved in host defense against infection and regulates the innate and acquired immune response.
Shipping and Storage
This product is shipped with dry ice. Avoid repeated freezing and thawing. Store for 4 weeks at 2-8°C, for 12 months at -80°C.
Documents
The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.
User Note
- Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute in 100 mM NaHCO3, 500 mM NaCl (pH 8.3) to a concentration of 0.1-1.0 mg/mL. Aliquote the reconstituted solution to minimise freeze-thaw cycles.
References
- Ihim, S. A. et al. Interleukin-18 cytokine in immunity, inflammation, and autoimmunity: Biological role in induction, regulation, and treatment. Frontiers in immunology. 2022, 13: 919973.