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Recombinant Bacillus cereus Endonuclease GajA (gajA), N-His

Cat. No.VG9C133

Product TypeVeterinary Antigens

Size

Product Overview

BioVenic's Recombinant Bacillus cereus Endonuclease GajA (gajA), N-His is a recombinant protein expressed from E.coli. Its predicted molecular weight is 28.8 kDa. The purity is>90% (SDS-PAGE).

Specifications

Type Recombinant Protein
Species Bacterium
Expression System E.coli
Purity >90% (SDS-PAGE)
Predicted Molecular Weight 28.8 kDa
Physical State Lyophilized
Formulation The buffer before lyophilization is a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.

Target Information

The primary role and function of Bacillus cereus Endonuclease GajA (gajA) in the field of veterinary medicine is as part of the bacterial defense system, particularly against phage invasion. GajA is a sequence-specific DNA-cleaving enzyme that is active in the presence of Mg2+, and its activity can be completely inhibited by high concentrations of NTP and dNTP. This indicates that GajA plays an essential role in the Gabija immune system by regulating phage infection through sequence-specific DNA cleavage, which is crucial for protecting bacteria from phage attacks.

Protein Bacillus cereus Endonuclease GajA (gajA)
Protein Synonym Gabija protein GajA; Nicking endonuclease GajA
UniProt ID J8H9C1

Shipping and Storage

This product is shipped with dry ice. Avoid repeated freezing and thawing. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use.

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

User Note

  1. Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.Aliquote the reconstituted solution to minimise freeze-thaw cycles.
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