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Mouse Anti-Swine CD172a Monoclonal Antibody

Cat. No.VD10H30

Product TypeAnimal-targeted Antibodies

Size

Product Overview

O12H30 monoclonal antibody is specific for Swine CD172a, also know as Swine SIRPA or BIT. It was purified by affinity chromatography on Protein A from tissue culture supernatant. It can be applied for Flow Cytometry (FC); Immunohistochemical (IHC); Immunoprecipitation (IP) and Western Blot (WB).

Specifications

Application FC; IHC; IP; WB
Clonality Monoclonal Antibody
Classification Primary Antibody
Clone O12H30
Host Mouse
Target Species Swine
Species Reactivity Swine
Specificity Swine CD172a
Isotype IgG1
Immunogen Swine alveolar macrophages
Purification Protein A Purified
Purity ≥ 90% (SDS-PAGE)
Concentration 1.0 mg/mL
Conjugation Unconjugated
Preservative and Stabilizer 0.09% Sodium azide (NaN3)
Buffer Phosphate buffered saline
Physical State Liquid

Target Information

CD172a, also known as signal-regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons. The gene coding for swine SIRPα has been mapped to porcine chromosome 17, in a region syntenic to the human chromosome 20 where SIRP genes have been mapped. CD172a acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 which plays an important role in involving in dendritic cell-mediated T cell activation, neutrophil migration, and phagocytosis.

Target Swine CD172a
Target Synonym SIRPA; BIT; CD172A; MFR; MYD-1; P84; PTPNS1; SHPS1; SIRP; Signal-regulatory protein alpha; signal regulatory protein alpha
Gene ID 494566
UniProt ID Q5K4Q3

Shipping and Storage

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt (up to 12 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

User Note

  1. Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

References

  1. Marquet, Florian, et al. "Characterization of dendritic cells subpopulations in skin and afferent lymph in the swine model." PloS one 6.1 (2011): e16320.
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