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Recombinant Swine Collagenase 3 (MMP13), N-His
Product Overview
BioVenic's Recombinant Swine Collagenase 3 (MMP13), N-His is a recombinant protein expressed from E.coli. Its predicted molecular weight is 20.1 kDa. The purity is>95% (SDS-PAGE).
Specifications
Target Information
Swine collagenase 3 is a proteolytic enzyme that efficiently cleaves native triple-helical type I collagen into characteristic 3/4 and 1/4 fragments. It is involved in the degradation of the extracellular matrix and plays a significant role in physiological processes such as tissue remodeling, inflammation, and development.
Shipping and Storage
This product is shipped with dry ice. It is recommended to aliquote as needed and store at -80°C upon receipt. Reconstituted protein solution can be stored at 4°C for 1 week, at < -80°C for 12 months.
Documents
The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.
User Note
- Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL.Aliquote the reconstituted solution to minimise freeze-thaw cycles.
References
- Uitto, V J. et al. Collagenase-3 (matrix metalloproteinase-13) expression is induced in oral mucosal epithelium during chronic inflammation. The American journal of pathology. 1998, 152,6: 1489-99.