Veterinary Therapeutic Protein and Peptide Bacterial Display Platform

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Veterinary Therapeutic Protein and Peptide Bacterial Display Platform

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Overview Bacterial Display Services Features Service Workflow Why Choose Us

Bacterial display is an advanced technique for engineering proteins and peptides, extensively utilized for their in vitro discovery. This method directly links the function of proteins or peptides to their encoding genes. At BioVenic, we create vast libraries containing billions of polypeptides displayed on the surface of bacteria. These libraries are meticulously screened to identify specific properties of candidates. Bacterial display can be specifically tailored to discover therapeutic proteins and peptides targeted to different diseases. Furthermore, bacterial display is a cell-based technology that provides high diversity and efficient screening of candidate molecules. Cell display systems offer high diversity and efficient screening of candidate molecules using various living cells, facilitate the discovery of high-affinity binders, and are versatile for various applications.

Bacterial Display Platform Overview

Bacterial display is a molecular technique applied to discover and screen present proteins and peptides on the surface of bacteria, typically E. coli. A gene encoding the desired protein or peptide is inserted into a plasmid vector that includes a gene for a surface protein. This plasmid is introduced into bacterial cells, leading to the expression and display of the protein or peptide on the bacterial surface. The typical bacterial display procedure for veterinary therapeutic proteins and peptides includes seven key steps.

Fig.1 The bacterial display selection cycle for veterinary therapeutic proteins and peptides is illustrated. (BioVenic Original) Fig.1 The veterinary therapeutic protein and peptide bacterial display selection cycle. (BioVenic Original)

Bacterial Display Services

	Description	Services
Library Construction	Diverse DNA sequences encoding various proteins or peptides are inserted into plasmid vectors. These plasmids are then transformed into E. coli MC1061 cells.	Help design and construct diverse protein and peptide libraries tailored to client's specific needs. Prepare plasmid libraries and transform them into bacterial cells.
Removal of Non-Specific Bacteria	The transformed bacteria are pre-incubated with normal (non-target) cells to identify and remove those bacteria that bind non-specifically.	Offer pre-incubation and screening services to remove bacteria that bind specifically to normal cells, enriching the library for specific binders.
Target Incubation	The remaining bacterial mixture is incubated with target cells or molecules.	Conduct incubation experiments to facilitate binding between the target ligand and the residual bacterial library. Optimize binding conditions to ensure maximum interaction between target ligands and displayed peptides or proteins.
Binding Analysis	The binding effects between the target cells and the bacteria are analyzed using fluorescence-activated cell sorting (FACS).	Provide FACS analysis to detect and quantify binding interactions between bacterial display candidates and target ligands. Label target ligands or bacteria with appropriate fluorescent markers for accurate detection.
Bacteria Sorting and Culturing	Bacteria that show binding to the target cells are sorted using flow cytometry. The sorted bacteria are then cultured in a growth medium to expand their numbers, ensuring a sufficient quantity for further analysis.	Sort the bacteria that bind to the target cells using flow cytometry. Post-sorting, we also culture the selected bacteria to expand the population of specific binders.
Subsequent Screening Rounds	Additional rounds of screening are performed to refine and enrich the library.	Perform multiple rounds of bacterial binding screenings to iteratively enrich for high-affinity binders. Optimize selection criteria throughout the screening rounds to ensure the best candidates are selected.
Isolation and Sequencing	The peptides or proteins displayed on their surfaces are then sequenced to identify the specific sequences that have demonstrated binding affinity.	Isolate DNA from the bacteria bound to the target ligands. Identify the peptides or proteins displayed on the bacterial surface, providing detailed analysis and reporting of the binding sequences.

Bacterial Display Platform Features

The ability to transform E. coli with large plasmid libraries allows for the screening of millions of different peptides and proteins simultaneously (10⁸ to 10¹¹).
Each displayed peptide or protein is directly linked to its encoding DNA within the bacterial cell, making it easy to identify.
This technology utilizes FACS for precise analysis and sorting of bacterial cells based on their binding affinities to target molecules.
The process can be iteratively repeated to enrich for the highest affinity binders, ensuring optimal selection of target-specific peptides or proteins.
Selection conditions can be precisely controlled and adjusted to favor the isolation of peptides or proteins with specific desired properties.

BioVenic also offers other cell-based display platforms, including yeast display platform, and mammalian cell display platform. Please get access to cell-based display platforms by the following links.

Service Workflow

At BioVenic, our bacterial display services for proteins and peptides start with a thorough consultation to understand your specific needs and project goals. We then design and construct a customized library of protein or peptide variants, incorporating advanced techniques to ensure diversity and efficacy. The next phase involves transforming bacteria with these constructs and utilizing bacterial display systems, to present the proteins or peptides on their surfaces. High-throughput screening is conducted to identify and isolate the most promising candidates. Following this, we perform a detailed analysis and characterization of the selected candidates to validate their performance and stability. Finally, we provide comprehensive reports that include data analysis and interpretation, along with recommendations. Our services are supported by ongoing consultation and feedback to ensure that your objectives are fully met.

Fig.2 The workflow for the service of veterinary therapeutic protein and peptide bacterial display is presented. (BioVenic Original) Fig.2 The service workflow of veterinary therapeutic protein and peptide bacterial display. (BioVenic Original)

Why Choose Us?

Expertise in mRNA Display

Our team specializes in an mRNA display platform designed for veterinary applications. We excel in discovering therapeutic proteins and peptides to advance animal health.

Expertise in Bacterial Display

Our team specializes in bacterial display technology for veterinary therapeutic protein and peptide development.

Independent Reaction Vessels

Independent Reaction Vessels Each bacterial cell acts as an individual reaction vessel, preventing cross-contamination and ensuring the independent evolution of peptides or proteins.

Comprehensive Service Offering

We offer a full range of services, from initial consultation and project design to execution, analysis, and reporting.

BioVenic provides comprehensive services of bacterial display, helping customers create high-quality products for animal health. For inquiries or additional information regarding our services, please do not hesitate to contact us. We are resolutely committed to providing expert support for your research and collaborating with you in advancing the field of veterinary therapeutic protein development.