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Veterinary Therapeutic Protein and Peptide High Throughput Screening
High-throughput screening (HTS) is a critical drug discovery technique widely utilized in the pharmaceutical industry for large-scale and rapid drug screening. Its applications have expanded to include the rapid identification and evaluation of numerous potential therapeutic protein and peptide molecules for veterinary purposes. The key goal of HTS is to identify 'hits' or 'leads'—compounds that influence the target in the desired way—through the screening of various compound libraries. This process typically utilizes liquid handling systems, robotic automation, plate readers for detection, and specialized software for instrumentation control and data analysis. BioVenic has established a cutting-edge HTS platform and offers comprehensive services tailored to the discovery and development of veterinary therapeutic proteins and peptides for preclinical research. Our expertise covers a wide range of species, including livestock, poultry, aquaculture, companion animals, and economically important insects. We also offer customized services of HTS tailored to your specific requirements to better meet customer needs.
Protein and Peptide HTS Assay Services
BioVenic has a comprehensive HTS assay platform for veterinary therapeutic protein and peptide discovery, with a strict quality control process to ensure effectiveness and efficiency. Our system is capable of preparing, incubating, and analyzing numerous plates at the same time, significantly reducing the time scale of protein and peptide discovery, as well as minimizing the labor required, and accelerating the data-collection process. Below are some of the services offered within our HTS assay system.
HTS Assay Methods | Description | Services |
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Cell-based Assays |
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Enzyme Activity Assays |
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Molecular Binding Assays |
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Protein-protein Interactions |
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Gene Expression Screening |
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Compound Library Screening |
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Protein and Peptide HTP Technologies
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Microarrays
Peptide microarray screening involves arranging thousands to millions of different short-chain peptides on a fixed surface, while protein microarrays contain a large number of different proteins. These microarrays are used to test the interactions of peptides or proteins with other molecules. BioVenic offers veterinary therapeutic protein and peptide microarray screening services, utilizing high-throughput screening to rapidly filter candidate molecules and identify proteins and peptides with specific biological activities.
Fig.1 Protein microarrays for protein-protein/peptide molecules interaction study. (BioVenic Original)
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Immobilization
The choice of material used as a solid support is one of the critical determinants of array performance. BioVenic provides common surfaces such as nitrocellulose membranes and polyvinylidene difluoride (PVDF) membranes for microarray fabrication that immobilize proteins and peptides and maintain their conformation. For the preparation of arrays with already synthesized proteins or peptides, there are generally three methods for immobilizing proteins/peptides, including physical adsorption, chemical reaction, or biological interaction. BioVenic offers all three approaches to immobilize veterinary therapeutic proteins and peptides, supporting high-throughput screening processes.
Fig.2 Immobilization methods for peptide array.1,2
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Other technologies
BioVenic also provides other advanced technologies in HTS for identifying veterinary proteins and peptides, including fluorescence resonance energy transfer (FRET), enzyme-linked immunosorbent assays (ELISA), and mass spectrometry (MS), surface plasmon resonance (SPR), high-content screening (HCS). These techniques collectively facilitate the thorough evaluation of therapeutic candidates by assessing their biological activity, binding affinities, and functional properties, thereby accelerating the discovery and development of veterinary therapeutics for diverse applications including livestock, poultry, aquaculture, companion animals, and economic insects.
High Throughput Screening Service Workflow
Our technology platform encompasses target-based HTS, receptor-based HTS, multiple screening models, and extensive libraries of compound samples and activity data. After consultation, we identify targets based on the client's specific needs. Our experts then design customized biochemical HTS assays according to different targets or receptors. Following this, a comprehensive hit screening using our compound libraries is performed to discover candidate proteins or peptides with high efficacy and stability. Finally, we conduct detailed analyses and provide a thorough report to the client.
Fig.3 The service workflow of therapeutic protein and peptide discover high-throughput screening platform. (BioVenic Original)
Why Choose Us?
Utilizes advanced high throughput screening technology to rapidly process large quantities of samples, significantly improving the efficiency and speed of screening veterinary therapeutic proteins and peptides.
Microarray technology boasts high sensitivity and specificity, ensuring that even very low abundance proteins and peptides can be accurately detected and analyzed.
Offers a variety of high throughput screening assay services, including cell-based screening and molecular binding assays, to accommodate different types of targets.
High-throughput screening employs rapid and sensitive detection devices to analyze a large number of samples simultaneously. BioVenic offers HTS services for veterinary therapeutic protein and peptide discovery, identifying biologically active candidates from an extensive library. Our service is designed to accelerate the discovery and development of new drugs for preclinical research, particularly for treating various veterinary diseases. If you need these services, don't hesitate to reach out to us.
References
- Nimse, Satish Balasaheb, et al. "Immobilization techniques for microarray: challenges and applications." Sensors 14.12 (2014): 22208-22229.
- Image retrieved from Figure 1 "Immobilization techniques for fabrication DNA microarray". Nimse, Satish Balasaheb, et al., 2014, used under CC BY 4.0, the title was changed to "Immobilization methods for peptide array".