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Veterinary Therapeutic Protein and Peptide Yeast Display Platform

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Overview Yeast Display Services Features Service Workflow Why Choose Us

Yeast display is a sophisticated cell-based tool that expresses proteins or peptides on the yeast surface after translation in a eukaryotic system. It is extensively used for the discovery and development of veterinary therapeutic proteins and peptides. Building on this, BioVenic has developed a unique yeast display platform specifically designed for the isolation and engineering of veterinary therapeutic proteins and peptides for therapeutic applications. We are dedicated to supporting our clients by identifying new proteins and peptides tailored to meet the specific challenges of animal health.

Yeast Display Platform Overview

Yeast display is a new method broadly applied to express the proteins or peptides at the surface of yeast cells. It surpasses phage display and other technologies due to its eukaryotic expression environment, which allows for precise tuning, including proper folding, modification, and translocation before the proteins or peptides are displayed on the surface. Consequently, it is considered a valuable alternative for obtaining recombinant proteins and peptides with high specificity for various targets or receptors.

Fig.1 The yeast display platform for veterinary therapeutic proteins and peptides. (BioVenic Original)Fig.1 The veterinary therapeutic protein and peptide yeast display platform. (BioVenic Original)

Yeast Display Services

Description Services
  • Construction of DNA Library
  • Clon a diverse array of gene fragments, each encoding different proteins or peptide sequences, into yeast-compatible expression vectors.
  • These vectors are designed to function within yeast cells, allowing for the expression and display of a broad spectrum of proteins or peptides.
  • Design and synthesize gene constructs encoding veterinary-specific proteins or peptides.
  • Construct a diverse DNA library with extensive variation to capture a wide range of potential candidates.
  • Yeast Cell Transformation
  • The constructed vectors are introduced into yeast cells through transformation techniques.
  • Optimize the protocols that transform constructed vectors into yeast cells (such as lithium acetate or electroporation).
  • Use or develop yeast strains optimized for high-efficiency expression and stability of veterinary proteins or peptides.
  • Protein/peptide-Displaying Yeast Library
  • The yeast cells are cultivated to display the expressed proteins or peptides on their outer membrane.
  • Implement and optimize yeast display systems to ensure effective surface presentation of the proteins or peptides.
  • Analysis System of the Displayed Protein/peptide
  • To evaluate the interactions and functionality of the displayed proteins or peptides.
  • Provide service for assessing functional properties such as activity or specificity relevant to veterinary applications.
  • High Throughput Screening by FACS or Yeast Cell Chip
  • High-throughput screening methods are employed to identify and isolate yeast cells that display proteins or peptides with the desired properties.
  • Utilize FACS or yeast cell chips for high-throughput screening to identify candidates with desirable properties.
  • Analyze screening data to select and isolate the most promising candidates.
  • Analysis of Deduced Amino Acid Sequence
  • Analyze the amino acid sequences of the isolated protein or peptide variants.
  • Perform sequencing of isolated candidates and analyze the deduced amino acid sequences.
  • Provide detailed insights into the structure-function relationships and potential applications of the proteins or peptides.

Yeast Display Platform Features

  • The yeast display platform owns libraries with large diversity sizes.
  • Proteins and peptides are displayed on the surface of yeast cells, facilitating direct interaction with target molecules and enabling functional assays in a biologically relevant context.
  • The yeast display system can be adapted to various types of peptides and proteins, including antibodies, antibody fragments, and other therapeutic proteins, making it suitable for diverse applications.
  • Allows for quantitative measurement of binding affinities and functional properties, providing detailed information on candidate performance.

BioVenic also offers other cell display platforms, including bacterial display platform, and mammalian cell display platform. You can also view our cell-based platforms by the following links.

Service Workflow

At BioVenic, we provide a comprehensive and integrated service for the development of veterinary therapeutic proteins and peptides using the yeast display platform. Following an initial consultation, we assist clients in designing and constructing specialized libraries. Our services extend to screening proteins and peptides, followed by an in-depth analysis of the candidates. We conclude with detailed reporting to ensure clients receive clear and actionable insights for their development needs.

Fig.2 The workflow diagram of the veterinary therapeutic protein and peptide yeast display platform services. (BioVenic Original)Fig.2 The service workflow of veterinary therapeutic protein and peptide yeast display platform. (BioVenic Original)

Why Choose Us?

BioVenic offers a comprehensive, one-stop solution tailored to meet the specific needs of clients, focusing on the development of veterinary biological macromolecular drugs. We apply matured yeast display technology to discover therapeutic proteins and peptides for animal health. Yeast display is a crucial cell-based technology for discovering and screening proteins and peptides. If you have any questions about this technology, please feel free to contact us.

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