Veterinary Therapeutic Protein and Peptide mRNA Display Platform

R&D Solutions

Get a quote

We're excited to learn more about your project and provide you with a customized quote tailored to your needs. Please fill out the form below, and we'll get back to you as soon as possible.

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Veterinary Therapeutic Protein and Peptide mRNA Display Platform

Inquiry Now

Overview mRNA Display Services Features Service Workflow Why Choose Us

mRNA display is an advanced in vitro screening method utilized to identify functional proteins or peptides from natural or synthetic DNA libraries. This technique involves covalently linking proteins or peptides to their encoding RNA, allowing for direct amplification of the protein. BioVenic specializes in discovering therapeutic proteins or peptides for veterinary applications using mRNA display technology. Our process includes generating mRNA-protein fusion libraries, conducting in vitro selection, and regenerating selected sequences. We are dedicated to supporting our clients in developing novel protein- and peptide-based therapies tailored to animal health needs, ensuring precision and effectiveness.

mRNA Display Platform Overview

The mRNA display is a tool for generating a diversity of protein and peptide libraries (ranging from thousands to trillions of unique sequences) for in vitro selection and direct evaluation. At BioVenic, we offer a specialized mRNA display platform for clients to discover therapeutic and novel proteins and peptides targeting various animal diseases. This method selects and evolves polypeptides with specialized functional properties through four main steps. At the end of this process, the library will be enriched with functional sequences. Cloning and sequencing will identify the selected functional proteins or peptides. mRNA display facilitates the discovery of new functional proteins or peptides without the need for prior design.

Fig.1 The cycle of ribosomal display at BioVenic for the discovery of veterinary therapeutic proteins and peptides. (BioVenic Original) Fig.1 The veterinary therapeutic protein and peptide mRNA display selection cycle. (BioVenic Original)

mRNA Display Services

	Description	Services
Transcription and Ligation	A double-stranded DNA library containing randomized nucleotide sequences is transcribed into an mRNA library, which is then ligated to a DNA fragment with puromycin attached to its 3' end.	Assist clients with the design and synthesis of customized double-stranded DNA libraries with randomized nucleotide sequences. Provide quality control and validation of DNA library services to ensure sequence diversity and integrity. Offer library design to optimize targeting properties and applications for specific animal diseases.
Transcription and Purification	The ligated mRNA Library is then translated in vitro. As ribosomes read RNA and not DNA, the ribosome stalls at the mRNA-DNA interface.	Supply optimized in vitro translation systems tailored to different mRNA libraries. Monitor and analyze the efficiency and fidelity of translation in real time. Troubleshoot and optimize translation conditions to maximize output and minimize stalling.
Selection (Affinity)	The fusion library undergoes a selection process, typically via co-immunoprecipitation with a target bound to beads, to remove proteins with low affinity for the target.	Develop and implement the co-immunoprecipitation systems using client-specified targets bound to beads. Conduct high-throughput screening to identify high-affinity interactions. Perform data analysis and report on selection efficiency, including providing recommendations for process refinement.
RT-PCR	Proteins that bind to the beads are reverse-transcribed into a new DNA library, which forms the basis for subsequent selection rounds.	Conduct reverse transcription of selected proteins back into DNA for generating new libraries. Refine and enhance libraries iteratively, based on selection outcomes.

mRNA Display Platform Features

mRNA display can generate libraries containing trillions of unique protein and peptide sequences.
Each peptide or protein is physically linked to its encoding mRNA, allowing for immediate identification of the sequence following selection.
This technology eliminates the need for living cells since the entire selection process occurs in a test tube.
Successful sequences from each selection round can be immediately amplified and subjected to additional rounds of selection.
The technology can be scaled up or down easily, and the selection conditions can be extensively modified.

BioVenic also applies other cell-free systems to discover therapeutic proteins and peptides, including the ribosomal display, and DNA display. Please find the following links.

Service Workflow

BioVenic specializes in providing comprehensive services for the development of veterinary protein and peptide drugs using mRNA display technology. This cutting-edge approach enables the discovery and optimization of high-affinity ligands with potential therapeutic applications. Our services encompass consultation, mRNA display library construction, screening, analysis and reporting, and after-sales support. Additionally, BioVenic offers a one-stop solution for the development of veterinary therapeutic proteins and peptides through its extensive display services, tailored to meet the unique needs of our clients.

Fig.2 The service workflow of veterinary therapeutic protein and peptide ribosomal display. (BioVenic Original) Fig.2 The procedural framework for mRNA display services in veterinary therapeutic protein and peptide discovery. (BioVenic Original)

Why Choose Us?

Expertise in mRNA Display

Our team specializes in an mRNA display platform designed for veterinary applications. We excel in discovering therapeutic proteins and peptides to advance animal health.

Simplified Genotype Identification

Direct linkage of proteins/peptides to their mRNA simplifies genotype identification, reducing the time required for discovery to validation and eliminating the need for further decoding steps.

Controlled, Customizable Selection Conditions

The cell-free system permits selections under controlled, customizable conditions that can mimic various physiological or pathological states, enhancing the real-world applicability of the proteins/peptides.

Versatility for Various Applications

The flexibility of mRNA display makes it suitable for both high-throughput industrial applications and targeted, specialized research projects.

Comprehensive Service Offering

We provide a full range of services from initial consultation and project design to execution, analysis, and reporting, ensuring comprehensive support throughout protein/peptide discovery process.

Advanced Technology and Facilities

We own state-of-the-art technologies and facilities for veterinary therapeutic protein and peptide discovery, which ensures high-quality and reliable results.

BioVenic offers an innovative mRNA display system for the discovery of veterinary therapeutic proteins and peptides. Our solutions help customers overcome challenges in the protein and peptide discovery process. For inquiries or more information about our services, feel free to reach out to us. We are dedicated to providing expert support for your research and collaborating to advance the field of veterinary therapeutic protein and peptide development.