Mouse Anti-Sheep Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Monoclonal Antibody

GAPDH, or glyceraldehyde-3-phosphate dehydrogenase, is a key enzyme in the glycolytic pathway, catalyzing the oxidation of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. This reaction is a crucial step in energy production through glycolysis, providing substrates for the Krebs cycle and the electron transport chain, ultimately generating ATP. GAPDH is highly conserved across different species, from bacteria to humans, indicating its essential role in cellular metabolism. Due to its ubiquitous expression and consistent levels in various tissues, GAPDH is commonly used as a loading control in molecular biology experiments, such as Western blotting and RT-qPCR, to normalize for variations in sample quantity and quality.