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Goat Anti-Renibacterium salmoninarum Polyclonal Antibody
Product Overview
BioVenic goat polyclonal antibody is specific for Renibacterium salmoninarum. It can be applied to ELISA of Renibacterium salmoninarum.
Specifications
Target Information
Renibacterium salmoninarum is a Gram-positive bacterium that is the causative agent of bacterial kidney disease (BKD) in salmonid fish, including trout and salmon. This intracellular pathogen is known for its ability to survive and replicate within the host's phagocytic cells, particularly in the kidney and spleen, leading to inflammation and organ damage. The bacterium's resistance to the host's immune response and its adaptation to the aquatic environment make it a challenging target for treatment and eradication. Diagnosis of BKD typically involves the isolation of R. salmoninarum from affected tissues, followed by biochemical tests and molecular identification, such as PCR.
Documents
The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.
User Note
- Two methods for rehydration and storage. Procedure A: Rehydrate with 1 mL of 50% glycerol. Antibody concentration will then be 1.0 mg/mL. The rehydrated product is stable for a minimum of 1 year at 2-8°C. Procedure B: Prepare immediately before use: 0.01M acetic acid solution and 0.177M carbonate-bicarbonate solution. Add 0.1 mL of 0.01M acetic acid solution to the antibody. Rotate vial until totally dissolved. Add 0.1 mL of the carbonate-bicarbonate solution and rapidly mix to obtain a clear or opalescent solution. Heat at 37°C for 30 minutes and allow to slowly cool to room temperature. Final concentration is 5.0 mg/mL. It should be stored at -20°C and is stable for a minimum of 1 year.
References
- Delghandi, Mohammad Reza, et al. "Renibacterium salmoninarum-the causative agent of bacterial kidney disease in salmonid fish." Pathogens 9.10 (2020): 845.