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Animal Mesenchymal Stem Cell Directed Differentiation
In the field of animal stem cell research, artificial control of cell fate is a critical factor that determines the extent and scope of its application. It is also one of the most important technical issues that researchers need to solve. BioVenic has a strong research background in animal mesenchymal stem cells (MSCs) and a well-established technical service system. This enables customers to gain a thorough understanding of the biological mechanisms and factors that influence cell differentiation into internal and external lineages. In order to meet the diverse needs of customers for the transformation of stem cells into somatic cells, our expert technical team provides the development and implementation of customized MSC differentiation solutions by delving into the genetic mechanisms of differentiation, signature products secreted by the cells, and positive stimulation.
Fig.1 Main Differentiation Direction of Animal Mesenchymal Stem Cells.1,2
Differentiation Potential of Animal MSCs
| Category | Direction | Evidence |
| Intralineage Differentiation | Osteogenesis | Confirmed by detecting alkaline phosphatase activity and calcium ion concentration. |
| Chondrocytes | Confirmed by detecting the expression of targeted genes that differentiate MSCs into chondrocytes and produce type II collagen and other markers. | |
| Muscle Cells | Confirmed by observation of cell morphology, expression of specific troponin 1, and blood vessel formation. | |
| Fat Cells | Confirmed by detection of fat droplets in cells after induction of adipogenesis. | |
| Extralineage Differentiation | Islet-like Cells | Confirmed by morphological observation and detection of insulin and nestin. In addition, the ability of differentiated cells to lower glucose levels was demonstrated. |
| Epithelial Cells | Confirmed by positive BrdU and cytokeratin staining. | |
| Nerve Cells | Confirmed by detection of neuronal cell markers such as neuron-specific enolase, microtubule-associated protein, and glial fibrillary acidic protein. |
BioVenic's directional differentiation technology services for animal MSCs include not only typical induced differentiation into adipocytes, osteoblasts, and chondrocytes, but also differentiation into neurons, hepatocytes, pancreatic islet cells, epithelial cells, and more. We are dedicated to developing and executing first-class directional induction solutions for researchers with advanced technology platforms and skilled experimental staff to effectively meet a wide range of research needs.
Cell Culture System Regulation
BioVenic has a comprehensive technology platform for the development of animal cell culture systems, including custom culture systems to direct the cell fate of MSCs. We meticulously prepare the medium composition and key parameters during the culture process to precisely control the differentiation process of MSCs into different lineages while ensuring cell viability. Our stem cell laboratory enhances the differentiation tendency of MSCs into specific somatic cells by adjusting the formula ratio and culture parameters. We use 3D culture and scaffold systems, co-culture with primary cells, and other methods to effectively direct cell fate.
Internal and External Factor Stimulation
BioVenic has introduced a range of induced differentiation solutions for scientific researchers worldwide. We guide animal MSCs to enhance the differentiation process in the desired direction by providing internal or external cellular stimulation signals. Our team of experts draws on their knowledge and experience of the key factors that influence the differentiation of MSCs into different somatic cells. This enables them to effectively achieve targeted induction that aligns with our client's research goals. We distinguish between the effects and mechanisms of signals delivered by MSCs of different species and origins on the growth environment, including the biophysical conditions that determine cell fate. This supports our comprehensive development of rational and reliable experimental designs.
Why Choose Us?
Expertise in Stem Cell Field
BioVenic has a long history of research in the field of animal stem cells and has professional technology and extensive experience in inducing the differentiation of MSCs.
Integration of Technology Platforms
BioVenic has mastered a comprehensive range of cutting-edge technologies related to animal cells, including three-dimensional culture, gene editing, culture system development, and cell characterization.
Diversification of Solutions
BioVenic develops specific induction methods and detection solutions for MSCs from different species and origins to meet the needs of scientific researchers.
BioVenic has professional technology, knowledge and rich practical experience in directed differentiation of animal stem cells. Based on the mature animal stem cell technology platform, we can provide a variety of experimental services to explore the differentiation potential of animal MSCs. We are proficient in a variety of cell markers used for cell characterization and verification to ensure that differentiation results are thoroughly and reliably confirmed. If you need research assistance in the field of directed differentiation of animal MSCs, please don't hesitate to contact us!
References
- Image retrieved from Figure 3 "Possible origin of mesenchymal stem cells and their differentiation into mesodermal, endodermal and ectodermal cells" Dias et al., 2019, used under [CC BY 4.0] (https://creativecommons.org/licenses/by/4.0/). The original title was changed to "Main Differentiation Direction of Animal Mesenchymal Stem Cells."
- Dias, Inês Esteves, et al. "Mesenchymal stem cells therapy in companion animals: useful for immune-mediated diseases?." BMC veterinary research 15 (2019): 1-14.