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Goat Anti-Borrelia burgdorferi Polyclonal Antibody
Product Overview
BioVenic goat polyclonal antibody is specific for Borrelia burgdorferi. It can be applied to ELISA of Borrelia burgdorferi.
Specifications
Target Information
Borrelia burgdorferi is a Gram-negative, spiral-shaped bacterium that causes Lyme disease, a multisystemic illness affecting humans and animals. This pathogen is transmitted primarily through the bite of infected black-legged ticks, making it the most common vector-borne disease in the northern hemisphere. The bacterium's surface proteins, such as outer surface protein A (OspA) and outer surface protein C (OspC), are crucial for its survival and pathogenicity. OspA is involved in the initial attachment to the tick's gut, while OspC helps the bacterium evade the host's immune system during transmission.
Documents
The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.
User Note
- Two methods for rehydration and storage. Procedure A: Rehydrate with 1 mL of 50% glycerol. Antibody concentration will then be 1.0 mg/mL. The rehydrated product is stable for a minimum of 1 year at 2-8°C. Procedure B: Prepare immediately before use: 0.01M acetic acid solution and 0.177M carbonate-bicarbonate solution. Add 0.1 mL of 0.01M acetic acid solution to the antibody. Rotate vial until totally dissolved. Add 0.1 mL of the carbonate-bicarbonate solution and rapidly mix to obtain a clear or opalescent solution. Heat at 37°C for 30 minutes and allow to slowly cool to room temperature. Final concentration is 5.0 mg/mL. It should be stored at -20°C and is stable for a minimum of 1 year.
References
- Sapi, Eva, et al. "Characterization of biofilm formation by Borrelia burgdorferi in vitro." Plos one 7.10 (2012): e48277.