This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Animal MSC-derived Extracellular Vesicle Analysis
Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have become the focus of basic research in animal cell and veterinary molecular therapy in recent years. Research focuses on the therapeutic effects and potential mechanisms of MSC-EVs in animal aplastic diseases. BioVenic supports multidisciplinary collaboration to advance projects related to MSC-EVs and provides customers with one-stop customized analytical services. From the production, separation, purification, and identification of extracellular vesicles, our laboratory technicians standardize the entire technical process to ensure that the research is completed under the guarantee of a rigorous scientific system. With our extensive scientific research background in the field of animal stem cells and the detection and analysis of animal cells and cell-related secretions, we are committed to providing customers with solutions that can effectively unravel the mysteries of MSC-EVs.
Background of MSC-EVs
During the in vitro culture process, the proliferative capacity of animal mesenchymal stem cells (MSCs) gradually decreases as the number of passages increases. This is accompanied by a certain degree of differentiation and even senescence, which may affect their regulatory efficacy and therapeutic potential in the fields of cell therapy and veterinary research. Second, the uncontrolled self-renewal capacity of stem cells may lead to tumorigenicity. The therapeutic effect of animal mesenchymal stem cells mainly depends on their paracrine effect, and MSC-EVs are the major paracrine products of mesenchymal stem cells. They contain various proteins and small RNA molecules. MSC-EVs facilitate intercellular communication, preserve the functions of the original cells, and exhibit low immunogenicity. Therefore, they offer extensive and promising therapeutic applications, surpassing cell therapy in several advantages.
Fig.1 Biological Process of MSC-EV Generation and Effect.1,2
Biogenesis of MSC-EVs
BioVenic specializes in custom research services related to the crucial process of extracellular vesicle (EV) biogenesis. Our expert team investigates EVs bearing different surface markers based on their cell sources and secretion mechanisms. In particular, we focus on variations in lipids that influence their structure, function, and biogenesis frequency. By analyzing the EV generation process, we can understand the life activities of the source cells and their impact on the biological functions of the effector cells. These effects include cell differentiation, transcription, cell proliferation, and immune regulation.
| Category | Size | Density | Feature |
| Exosomes | 40-100 nm | 1.13 g/mL - 1.19 g/mL | Occurs via the endocytosis-extraction pathway and is released via efflux regulated by p53. It is controlled by cytoskeletal activation pathways but is not affected by calcium. |
| Microvesicles | 100-1000 nm | 1.04 g/mL - 1.07 g/mL | Formed by budding from the outside of the cell membrane, this process involves cytoskeletal reorganization and is also dependent on intracellular calcium ion concentration. |
Isolation of MSC-EVs
BioVenic develops cell culture conditions and separation methods suitable for the isolation of extracellular vesicles to enhance research on paracrine signaling of mesenchymal stem cells and exosomes. We adopt different separation methods according to the source and characteristics of MSC-EVs. By strictly controlling collection procedures, time, and sample processing methods, we ensure efficient, reliable, and consistent results.
| Methods | Contents | Advantages |
| Ultracentrifugation | Use submicron filters or low-speed centrifugation to remove cell debris, microvesicles, or apoptotic bodies. Ultracentrifugation is used to remove large vesicles and isolate exosomes. Remove the supernatant and resuspend the exosomes in a relatively small volume of buffer. |
|
| Polymer Precipitation | Incubate the polymer precipitant with the sample at 4°C. Collect the pellet after low-speed centrifugation and resuspend the exosomes. |
|
| Size Exclusion Chromatography | Proteins are removed by using a porous material column. Exosomes are separated by elution. |
|
| Ultrafiltration | Samples are systematically separated by using polyether sulfone ultrafiltration membranes with different molecular weight cutoffs. |
|
| Immunoaffinity Capture | Exosome separation is achieved by specific binding of exosome-specific membrane antigens to antibodies using techniques such as enzyme-linked immunosorbent assay separation and antibody-coated immunomagnetic beads. |
|
| Microfluidic Chip | A transverse electric field forces the sample on the microfluidic chip out of the crossflow and into the agarose gel. This process filters out unwanted cell debris while the ion-selective membrane concentrates the exosomes by enrichment. |
|
Characterization of MSC-EVs
BioVenic is continuously improving the quantification and characterization methods of MSC-derived EVs based on practical experience. BioVenic provides customers with efficient, versatile, and highly compatible solutions for the characterization of MSC-EVs. Our customized services support both general and single vesicle characterization. General characterization typically focuses on specific protein markers using Western blot or ELISA. Single vesicle characterization requires imaging technology and biophysical characterization. We are committed to developing a standardized analytical platform for the comprehensive characterization of different EV subpopulations and the analysis of the molecular composition of the cargo transported in different types of EVs.
Technologies for Analysis and Characterization of Animal MSC-EVs
Why Choose Us?
EVs isolated from MSCs provide the same therapeutic benefits as stem cells while avoiding the immune-related issues associated with the use of whole stem cells.
By using our services to analyze EVs derived from animal stem cells, we are helping to understand their kinetics, mechanisms of action, and active ingredients to accelerate the development of animal drugs and cell-free therapies.
We help establish reliable, reproducible, and robust solutions and scientific systems for the generation, isolation, and purification of animal MSC-EVs.
BioVenic offers customized solutions for scientific research on animal mesenchymal stem cells and their secretome. Starting from a basic research perspective, we develop a comprehensive experimental design to identify variations in composition, quantity and efficacy among different subpopulations within a heterogeneous EV population. We also focus on the broad prospects of EVs in the veterinary field and promote the development of appropriate applications through detailed characterization of specific MSC-EVs. If you are interested in our services on the secretome of animal mesenchymal stem cells, please contact us for further discussion.
References
- Image retrieved from Figure 1 "Schematic representation of EVs biogenesis." Abreu et al., 2016, used under [CC BY 4.0] (https://creativecommons.org/licenses/by/4.0/). The original title was changed to "Biological Process of MSC-EV Generation and Effect."
- Abreu, Soraia C., Daniel J. Weiss, and Patricia RM Rocco. "Extracellular vesicles derived from mesenchymal stromal cells: a therapeutic option in respiratory diseases?" Stem cell research & therapy 7 (2016): 1-10.