Animal Cell Line Culture Development

R&D Solutions

Get a quote

We're excited to learn more about your project and provide you with a customized quote tailored to your needs. Please fill out the form below, and we'll get back to you as soon as possible.

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Animal Cell Line Culture Development

Inquiry Now

Flow Chart of Animal Cell Line Isolation and Culture Cell Line Culture Environment Control Cell Line Culture System Determination Cell Contamination Control Cell Contamination Control Comparison of Animal Primary Cells and Cell Lines Why Choose Us?

Different types of animal cell lines or primary cells are used in research across various fields, including adherent cells and suspended cells, each with unique growth habits. The establishment of animal cell lines is an essential skill for conducting in vitro research projects. Once the cells of animals are separated, the cells that initiate the first subculture of the primary culture are referred to as cell lines. Animal cell lines can be divided into finite cell lines and continuous cell lines. The latter have acquired unlimited reproductive capacity and can survive continuously. BioVenic develops animal cell line culture programs tailored to the specific needs of researchers to maintain the vitality and subculture status of cell lines in vitro. We focus on the cultural environment and system of animal cell lines, particularly emphasizing the matching and selection of culture media. Our goal is to assist customers in establishing appropriate in vitro culture models and acquiring high-quality cell lines.

Flow Chart of Animal Cell Line Isolation and Culture

Fig.1 Animal Cell Line Isolation and Culture. (BioVenic Original)

Cell Line Culture Environment Control

BioVenic designs and optimizes animal cell line culture programs based on the key parameters of the animal cell growth and maintenance environment. Our expert team has extensive practical experience in controlling the physical and chemical environment of cell reproduction, including temperature, pH, osmotic pressure, etc., as well as the physiological environment, such as hormones and nutrient concentrations. This precise parameter adjustment affects the growth state of the cell line. The culture medium for animal cell lines is the major growth environment for cells. We tailor high-quality and dependable culture programs for customers by gaining a deeper understanding of serum composition, identifying the necessary growth factors for the proliferation and passage of various cell lines, and optimizing the cell microenvironment during the culture process.

Cell Line Culture System Determination

BioVenic specializes in the development of two fundamental cell culture systems: adherent culture and suspension culture. Except for hematopoietic cell lines and some other cells, most mammalian cells are adherent-dependent and must be cultured on a suitable matrix. The matrix must be specially treated to allow cell attachment and spreading. Many cell lines can also be cultured in suspension, and most insect cell lines grow well in monolayer or suspension cultures. We determine the format and medium of cell growth and maintenance based on the specific experimental requirements related to the cell line in the customer's project.

Cell Contamination Control

BioVenic effectively addresses the common contamination issue during in vitro cell growth and maintenance in biological laboratories. In all cell culture-related services, we assist our clients in overcoming and preventing negative consequences associated with contamination, such as alterations in cell status, decreased reproducibility of experimental results, and potential cell death. We rigorously monitor contamination and ensure strict quality control of animal cells and related products. At the same time, we utilize the contamination control platform to detect and eliminate bacteria, fungi, and mycoplasma in the culture system. This ensures the correct pH, osmotic pressure, and an endotoxin-free environment.

Cell Contamination	Mycoplasma Contamination	Slow growth rate, decreased vitality, and easy detachment. Cell morphology changes. During the cultivation process, the culture medium quickly turns yellow, indicating a high concentration of invisible microorganisms multiplying in the culture liquid and consuming nutrients. Under the microscope, much debris is visible in the culture medium that is not turbid. Cells tend to aggregate.
	Bacterial Contamination	Under the microscope, a large number of spherical particles can be seen floating in the culture medium. The cell culture medium quickly becomes cloudy in a short period of time. The culture medium does not appear turbid when left standing, but a significant amount of turbid matter floats up when slightly shaken. Cells stop growing and die in large numbers.
	Fungal Contamination	Under a microscope, you can observe crisscrossing filamentous, dendritic, or tubular hyphae between cells or in the culture medium. Light yellow or white floating objects will form in the cell culture medium, which is visible to the naked eye, but the culture medium will not become cloudy in the short term. Most hyphae can be observed in chain-like arrangements under high magnification, while Candida and yeast strains appear oval and scattered on and around the cells.

How to Choose the Right Cell Line?

Species and Functional Characteristics

Based on the purpose of the project and experiment, select the appropriate cell line based on its function. For instance, cell lines derived from the liver and kidney may be more suitable for toxicity testing.

Finite or Continuous

While selecting a finite cell line provides more options for expressing normal functions, continuous cell lines are often easier to expand and maintain.

Growth Conditions and Characteristics

Cell lines should be selected based on criteria such as cell growth rate, saturation density, clone formation rate, and suspension growth ability.

Normal or Transformed

Transformed cell lines typically exhibit rapid growth, high seeding efficiency, continuous culture capability, and reduced serum dependency in the culture medium. However, their phenotype has been altered to achieve immortality through genetic transformation.

Others

Consider whether the cell reserve is sufficient, whether the characteristics of the cell line are clear, and whether validation is required. If an abnormal cell line is used, check if there is an equivalent normal cell line that can be used as a control.

Comparison of Animal Primary Cells and Cell Lines

Characteristics	Primary Cell	Cell Line
Proliferation	Weak	Strong
Reproduction	Generally, within 10 generations	About 50 generations or more
Genetic Integrity	Not changed	Changed
Biological Properties	Close to in vivo cell physiology	Deviate with the number of divisions
Cultivation	Relatively difficult	Easy

Why Choose Us?

The culture system development process has been specially optimized for animal cell lines to promote their in vitro growth and maintain cell viability.

The design of programs related to improving cell line culture medium and optimizing the culture environment is based on extensive experience and expertise in animal cell growth and maintenance projects.

The strict and standardized quality control system ensures the singleness and purity of animal cell lines during in vitro culture, reducing the risk of cross-contamination and mis-authentication.

BioVenic offers comprehensive development services for the in vitro culture of animal cell lines. We possess a profound understanding of the culture conditions and parameters for cells sourced from various origins. We tailor the cell line culture program to meet the specific project requirements and research objectives of our clients, whether it involves finite or continuous cell lines. Our customized services are designed to fully address a wide range of animal cell culture needs. For more detailed information on our cell line culture services, please do not hesitate to contact us.