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Animal Cell Invasion and Migration Assay
One way to evaluate animal cells is to detect cell motility, which refers to cell invasion and migration. Migration is a fundamental characteristic of living cells and plays a crucial role in normal development, immune response, and the progression of disease processes. Invasion experiments primarily assess the invasive potential of tumor cells. BioVenic provides a comprehensive solution for measuring animal cell motility. We also design and customize various service packages based on specific project requirements. Our detection solutions mainly include cell scratch experiments and Transwell cellular invasion or migration assays, combined with automated confocal imaging technology platforms to record the migration behavior of animal cells more intuitively and comprehensively. We are committed to providing powerful research tools for various disciplines in animal medicine, cell biology, bioengineering, and related fields.
Animal Cell Invasion and Migration Assay Services
Animal cell migration assays support the exploration and analysis of a variety of pathological and physiological processes, including wound healing, diseases, cell growth, and differentiation. Animal cell invasion assays often yield reliable data for studies related to the metastasis of animal tumor cells.
Transwell Assay
BioVenic's Transwell-detection experiment is a powerful tool primarily used to study the migration and invasion abilities of animal cells. The basic principle involves using a specialized device to assess how components in the lower culture medium affect cells in the upper chamber. This method allows for the study of how culture medium components influence cell movement, as well as the measurement of cellular invasion or migration abilities towards a chemical attraction gradient. We strategically optimize and pair polycarbonate membranes with varying pore sizes and treatments within the device based on specific experimental objectives to provide research services in co-culture, cell chemotaxis, cell migration, cell invasion, and other related areas.
Fig.1 A Schematic Diagram of the Trans-endothelial Migration Assay.1,2
Cell Scratch Assay
BioVenic offers personalized cell scratch assay services to researchers worldwide to assess cell migration and repair abilities. We have established a sophisticated experimental system, standardized technical procedures, and proficient scratching technology to guarantee our ability to detect the invasion and metastasis potential of adherent tumor cells for clients through a straightforward and efficient cell movement detection technology. This technical service extensively examines the influence of various factors, such as veterinary drugs and genes, on cell migration and repair.
Fig.2 Assessing Cell Migration Using a Scratch Wound Healing Assay.3,4
FAQs about Animal Cell Invasion and Migration Assays
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| The migratory movement of animal cells refers to the migration of cells from one location to another along a concentration gradient, propelled by chemical signals. Many physiological and pathological processes in animals, such as tissue repair, cell differentiation, embryonic development, and metastasis of animal tumor cells, rely on cell migration. Cell invasion involves the migration of cells from one location to another across the extracellular matrix or basement membrane matrix, driven by chemokine concentration gradients. Animal cell invasion occurs during the inflammatory response of normal cells and the metastasis of tumor cells. Therefore, research on this topic can explore the role and significance of potential invasion mechanisms in various physiological and pathological processes. |
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| The time required for migration experiments is usually shorter than that for invasion experiments. The results can typically be obtained within a few hours, whereas invasion experiments generally necessitate a longer duration, such as 24 hours or even 48 hours. The duration of migration or invasion experiments, however, is influenced by various factors, including the type and concentration of inducing factors, as well as the characteristics of the cells. It should be noted that the longer the incubation time of the migration experiment, the more likely the cells will spontaneously migrate. |
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| Suspension cells can be assayed for migration or invasion. Based on the Transwell-assay method, suspended cells will settle in the lower chamber after passing through the pores on the membrane under the influence of induction factors. We collect the cells in the receiving plate, count them, and quantify them using a hemocytometer, flow cytometer, or fluorescent staining. |
Why Choose Us?
Mature and strict standardized experimental procedures reduce errors caused by experimental operations.
Customize exclusive solutions for detecting cell migration or invasion in cells derived from various tissues, including healthy and cancerous cells.
Combining cutting-edge live cell real-time monitoring systems with traditional detection methods can lead to a more accurate characterization of the cell movement process.
BioVenic provides one-stop cell testing services for researchers in the animal cell-related research field, which includes evaluating and investigating cellular invasion or migration abilities. Our expert team specializes in designing customized solutions for detecting cell migration behavior, which are highly valuable research tools for scientific projects in animal medicine and cell engineering disciplines. BioVenic's high-throughput detection technology platform and fluorescence imaging live cell monitoring system enable us to offer customers more detailed, accurate, and real-time information and analysis. Please feel free to contact us to discuss with us about your cell motility testing research needs.
References
- Image retrieved from Figure 1 "The cancer cell-derived culture supernatant and the exosome-enriched fraction significantly enhance the transendothelial migration of breast cancer cells." Cen et al., 2019, used under [CC BY 4.0] (https://creativecommons.org/licenses/by/4.0/). The original image was modified by extracting and using only part B, and the title was changed to "A Schematic Diagram of the Trans-endothelial Migration Assay."
- Cen, Junyu, et al. "Exosomal thrombospondin-1 disrupts the integrity of endothelial intercellular junctions to facilitate breast cancer cell metastasis." Cancers 11.12 (2019): 1946.
- Image retrieved from Figure 1 "Representative images of scratch wound healing assay at 0, 12, and 24 h." Bahar and Hyonok, 2021, used under [CC BY 4.0] (https://creativecommons.org/licenses/by/4.0/). The original title was changed to "Assessing Cell Migration Using a Scratch Wound Healing Assay"
- Bahar, Entaz, and Hyonok Yoon. "Modeling and predicting the cell migration properties from scratch wound healing assay on cisplatin-resistant ovarian cancer cell lines using artificial neural network." Healthcare. Vol. 9. No. 7. MDPI, 2021.