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Animal Cytotoxicity Assay
Animal cytotoxicity analysis services focus on the detection and quantification of damage to animal cells under specific conditions. These assay methods are usually based on cell membrane damage. Toxicity assessment of animal cells is often used for the safety evaluation of veterinary drug compounds and the development of cell culture systems. BioVenic's animal cytotoxicity analysis service measures the ability of a specific substance or compound to damage or even kill cells. Our solutions include various in vitro methods or assays to assess cytotoxicity by examining the mechanisms of cell damage and death. Animal cell toxicity detection technology broadly supports basic research and veterinary drug discovery by screening animal cells to generate libraries of toxic compounds.
Classification of Analytical Methods for Animal Cytotoxicity
To meet individual and comprehensive research needs, BioVenic classifies animal cell cytotoxicity detection methods by analysis type, starting from the detection entry point and focus, making it easier for researchers to choose the solution that best meets their specific needs.
Dye Exclusion Analysis of Animal Cytotoxicity
BioVenic has introduced a mature animal cell dye exclusion test service to assess the amount of viable and healthy cells in a cell population. Our Dye Exclusion Service follows a standardized process that is optimized to account for common influencing factors that can lead to an erroneous assessment. These factors include the hysteresis of damage to cell membrane integrity and the likelihood that damaged cells will not accept the dye. This optimization is critical to ensure the scientific validity of the test results. Our solution requires a small number of animal cells, is efficient and convenient, and can detect cell death in undivided cell populations.
Colorimetric Analysis of Animal Cytotoxicity
Among BioVenic's solutions for cytotoxicity analysis, colorimetry is a common method used to estimate animal cell viability and cytotoxicity. The colorimetric method is designed to quantify biochemical indicators. The reagent reacts with living cells and changes color. This allows the spectrophotometer to perform colorimetric quantification of cell viability, which is used to estimate the vitality of animal cells.
Luminometric Analysis of Animal Cytotoxicity
BioVenic offers fluorescence and luminescence detection solutions for a thorough evaluation of cell viability and cytotoxicity. Our optical detection platform includes advanced fluorescence microscopes, fluorometers, fluorescence or luminescence microplate readers, and flow cytometers to ensure the professionalism and accuracy of our detection solutions.
Comparison of Animal Cytotoxicity Assays
| Assays | Principles | Features |
| MTT Detection Method | MTT is a hydrogen ion accepting compound. The dye is positively charged and can permeate the cell membrane. Succinate dehydrogenase in the mitochondria of living cells can reduce exogenous MTT to form water-insoluble dark blue MTT formazan crystals, whereas dead cells lack this ability. |
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| CKK-8 Detection Method | In the presence of electron coupling reagents, mitochondrial dehydrogenase can reduce it to highly water-soluble orange-yellow formazan. The color depth is directly proportional to cell proliferation and inversely proportional to cytotoxicity. Use a microplate reader to measure the OD value, which indirectly reflects the number of viable cells. |
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| LDH Release Method | Under normal circumstances, LDH cannot penetrate the cell membrane. When cells are killed, LDH is released outside the cells. Damaged cells can be quantified by detecting the amount of LDH released. |
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| Luciferase Method | The reaction between luciferase and substrate is dependent on the presence of active ATP in the cells, and viable cells are quantified by detecting the fluorescence produced by the cells. |
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| Live Cell Dye Method | The fluorescent dye used is a dye for living cells. When the labeled cells die, the fluorescence disappears. The number of cells is directly reflected by the detection of double positive cells. |
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Why Choose Us?
BioVenic comprehensively evaluates animal cytotoxicity using multiple detection methods to ensure the accuracy and repeatability of experimental results.
BioVenic specifically detects cell characteristics associated with cytotoxicity, such as membrane integrity, cell content, and cell metabolism.
BioVenic provides comprehensive customer service and professional technical support for one-stop cytotoxicity detection solutions.
BioVenic has a strong background in animal cell biology and molecular biology, along with extensive animal cell line resources and technology platforms.
BioVenic serves a variety of purposes for animal cytotoxicity assays, ranging from basic research and quality control to animal disease research. Our expert team designs and develops customized solutions for each specific purpose, along with the necessary readouts and results. In addition, we carefully select the appropriate experimental method for measuring animal cytotoxicity. Our live cell imaging technology easily provides valuable information about changes in cell number or morphology over time in a target animal. We are committed to providing an in-depth understanding of cellular health under specific conditions. Please contact us for animal cytotoxicity assays.