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Animal Genome Modification Validation by Sequencing
There has been a significant advancement in genome-editing tools, which has made it easier for researchers to introduce genetic modifications directly into animal embryos, which enhanced the simplicity, effectiveness, and the variety of genetic variations that researchers can access across various animal species to enhance animal breeding progress. BioVenic is dedicated to providing advances PCR, Sanger sequencing, and next-generation sequencing (NGS), as well as transcriptional and epigenomic profiling services for genome modification validation in the field of precision animal breeding.
Edits Validation by Sequencing
Our advanced sequencing platform offers a wide range of capabilities, from targeted gene sequencing to whole genome sequencing and RNA sequencing. This empowers researchers to validate CRISPR modifications like knock-ins, knockouts, and edits, while also delving into the analysis of CRISPR off-target effects, animal gene expression patterns, and even animal genome methylation. We're dedicated to providing comprehensive genome sequencing and data analysis support for your animal genome modification research needs.
| Animal Genome Modification Validation by Sequencing | |||
|---|---|---|---|
| Validation Method | Description | Qualitative and/or Quantitative | Identified Gene Editing Types |
| T7 Endonuclease 1 (T7E1) Assay | Detection of structural deformities in heteroduplexed DNA. | Semi- Quantitative | Indel and point mutation |
| On-target PCR and Sanger sequencing |
Spanning target region. Sequencing confirmation of allele in both directions. |
Qualitative + Quantitative | All editing types |
| Droplet digital PCR for copy number variation analysis | copy number analysis of excised region or insertion sites and HDR donor templates. | Qualitative + Quantitative | All editing types |
| Long read sequencing |
Detection of upstream, downstream events, and re-arrangements. Detection of repetitive regions of allele sequence. |
Qualitative + Quantitative | Larger Knock-ins |
| High-throughput NGS sequencing | Targeted sequencing or whole genome sequencing. | Qualitative + Quantitative | Genome-wide off-target effect and all editing types |
High-Throughput NGS for Edits Validation
NGS is the major method for conducting a comprehensive analysis of genetic modifications induced by CRISPR. It surpasses traditional techniques like Sanger sequencing, T7E1, and IDAA assays in terms of effectiveness and efficiency for screening edited cell lines. NGS excels due to its exceptional precision and consistency, allowing the detection of even subtle genetic changes at the intended genomic site and predefined off-target locations. Our recommended approach begins with selecting off-target sites and utilizing our CRISPR analysis system. We also provide a data analysis pipeline to precisely quantify both on-target and off-target edits.
Fig.1 Schematic diagram of the IDAA assay. (BioVenic Original)
Functional Sequencing Validation Services by BioVenic
We provide transcriptional and epigenomic sequencing methods, aiding researchers in assessing the impact of animal genome editing on the structure and function of animal genes.
| Functional Validation of Animal Genome Modification | |
|---|---|
| Validation Method | Description |
| RT-PCR and RNA-Seq | Transcriptional validation of animal genome modification. |
| ChIP-Seq | Validation of the effects of animal genome edits on DNA-protein binding. |
| Epigenomic sequencing and analysis | Examine the genome edits downstream influences on methylation patterns and chromatin remodeling. |
Our Advantages
BioVenic offers a comprehensive suite of advantages for precision animal breeding research.
- We empower researchers to select off-target sites and validate on-target and off-target edits.
- Our transcriptional and epigenomic profiling services help researchers assess the structure and function alteration of animal genes, supporting advancements in animal breeding.
- Our advanced sequencing platforms and a scientific team of bioinformatics and biostatistics allows for versatile genome editing analysis.
BioVenic provides not only precise NGS sequencing and Sanger sequencing services to accurately and efficiently detecting and validating animal modified alleles, but also offers a wild range of genome modification validation assays such as T7E1, and IDAA assays, post-transcriptional validation assays- Western Blot, etc. Please don't hesitate contact us to learn more and tell us about your project.