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T7 Endonuclease 1 (T7E1) Assay
The T7 endonuclease 1 (T7E1) mismatch assay is a wide-spread method for detecting genome editing events from CRISPR gene targeting. BioVenic relies on a professional team of experts to provide quality services based on the T7 endonuclease 1 assay. Our scientists are proud to be able to provide our customers with information and advice on the field of gene editing validation with our maximum knowledge and competence.
Introduction of T7 Endonuclease 1 (T7E1) Assay
T7 endonuclease 1 mismatch assay is an efficient and cost-effective method for estimating the relative gene editing efficiency in cell populations, or for screening various clones and conditions. T7 endonuclease 1 is a phage-derived mismatch-specific enzyme that cleaves mismatched cruciform DNA, heteroduplex DNA, branched DNA, and Holliday junctions. Following a gene editing experiment, the genomic DNA around the target site is amplified by PCR, and the PCR amplicon is denatured and re-annealed by heating and slow cooling. If a Non-homologous end joining (NHEJ) event occurs, multiple products may be generated upon reannealing. Heteroduplexes are formed when the WT strand is reannealed to an indel-carrying strand that results in a mismatch. The heteroduplex product with mismatches is cleaved by T7 endonuclease. Separation of the DNA product by gel electrophoresis after treatment with T7 endonuclease will produce a band pattern indicative of the number of heteroduplexes in the sample. The amount of cleaved heteroduplexes is directly related to the amount of indel activity.
Fig.1 Evaluation of genome editing using T7 Endonuclease 1.
Our T7 Endonuclease 1 Assay Services
Equipped with experienced scientists and an advanced gene editing validation platform, BioVenic is committed to providing T7 endonuclease 1 assay services to our customers in an efficient manner. We utilize unparalleled custom protocol design, advanced project lifecycle management, and real-time data to ensure ideal results.
- Validation Process Overview
Why Choose Us?
We have expertise in the field of gene editing validation and are able to develop customized solutions based on our clients' R&D needs. Our dedicated scientists will provide full technical and professional support throughout the duration of the project.
We have established a well-established and advanced gene editing validation platform to provide high-quality T7 endonuclease 1 assay services to ensure the reliability of your test results.
It's our attention to detail, scheduling of timelines, and keen project management that make us stand out from the rest. We are creative while paying close attention to the progress of your research project and your budget.
Want to Learn More?
BioVenic is committed to providing a full range of gene editing validation services following the ethics and regulations, including but not limited to genome sequencing, T7E1 assay, IDAA assay, TIDE assay, functional validation. We desire to leverage our advanced technology and extensive experience to help our customers break the bottleneck of gene editing technology in animal breeding and genetic applications. If you are interested in our services, please contact us for more information and a detailed quote.