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Virus-mediated Gene Delivery in Precision Animal Breeding
The CRISPR system has become a highly versatile genome editing platform in the field of molecular biology for introducing targeted genome modifications into mammalian cells. The implementation of genome editing requires the safe and effective delivery of functional components into target cells. Recombinant viral vectors can safely infect a variety of dividing and non-dividing cell types, and are commonly used as engineering vectors in animal genome editing. By combining CRISPR with virus-mediated delivery technology, BioVenic accelerates the research process of genome editing in farmed animals for customers and overcomes technical obstacles related to nucleic acid delivery.
Our Services
Viral delivery methods are currently the most attractive option for efficient gene delivery in vitro and in vivo. BioVenic offers the following customized services for commonly used viral vectors in animal genome editing tool delivery:
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Plasmid Construction and Purification
BioVenic relies on the mature CRISPR technology platform, high-throughput gene synthesis and sequencing platform, and seamless cloning vector construction technology to provide you with customized plasmid vector services for genome editing tools in farmed animals, including viral vector packaging, gRNA-related services, plasmid construction and purification, verification. -
Virus Packaging and Concentration
BioVenic offers service that transfects the constructed high-purity plasmid vectors into different packaging cells according to the type of viral vector. The process allows us to package and concentrate the virus particles. We apply technologies such as PEG concentration and density gradient centrifugation to deliver highly purified viruses according to the different levels of scientific research purposes of our customers and to ensure the quality and titer of viral vectors.
Viral Titering
Virus titer is a crucial indicator of the virus's infectivity. Preparation of impure plasmids, modification of inverted terminal repeats leading to mutations, toxicity of transfection, non-targeted cleavage of viral genomes by Cas9, and other factors can contribute to unsatisfactory titers. For this key indicator, BioVenic provides a comprehensive titration process for virus products to ensure a high titer of the viral vector and achieve effective gene transduction.
Quality Control (QC)
BioVenic conducts quality control tests for safety, including purity, sterility, and non-toxicity. Virtually all steps required for efficient gene delivery are optimized in our service offerings, including protection and transport of nucleic acids in the extracellular environment, internalization of nucleic acids, routing to the nucleus, and more. Additionally, BioVenic has achieved permanent modification of the host cell genome to meet specific scientific research goals proposed by customers.
Comparison of Viral Vectors
| Lentivirus (LV) | Adenovirus (AV) | Adeno-Associated Virus (AAV) | |
|---|---|---|---|
| Virus Genome | Two single-stranded linear RNAs | A double -stranded linear DNA | A single-stranded linear DNA |
| Envelope Structure | Yes | No | No |
| Tropism | Broad | Ineffective for some cells | Depending on viral serotype |
| Maximum Titer | High | Very high | High |
| Main Uses | Cell culture and in vivo | In vivo | In vivo |
| Immunogenicity | Low | High | Very low |
| Packaging Capability | ~ 6.4 kb | ~ 8.3 kb | ~ 4.7 kb |
| Integrated into Genome | Yes | No | Not always |
| Gene Expression | Long-term stable expression | High-level transient expression | Long-term expression |
| Advantages |
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Why Choose Us?
Efficient virus-mediated solution for gene delivery in precision animal breeding.
High titer, safe, and non-toxic, ready-to-use delivery results.
Virus packaging service with guaranteed efficiency in gene editing for animal genomes.
Highly customized customer service, professional and reliable technical support, and fast response.
BioVenic utilizes a well-established platform for editing the genome of farmed animals. Our commitment is to provide high-quality virus-mediated gene delivery services to scientific researchers in the field of precision animal breeding worldwide. We improve and optimize service content at multiple levels to resolve technical bottlenecks for you. If you are facing challenges with gene delivery in farmed animals that need to be resolved, please do not hesitate to contact us and customize your personalized service plan.
Reference
- Giacca, Mauro, and Serena Zacchigna. "Virus-mediated gene delivery for human gene therapy." Journal of controlled release 161.2 (2012): 377-388.