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DNA Microinjection in Precision Animal Breeding
Microinjection is a physical method that utilizes a microscope and a microneedle to inject the CRISPR system directly into specific locations within cells. It is a recognized delivery method with an efficiency close to 100%. BioVenic assists scientific researchers in overcoming the molecular weight limitation of viral vector cargo by offering tailored microinjection services. We directly penetrate the cell membrane and deliver the cargo into the cell, and in some cases, even into the nucleus. This approach addresses the demand for delivering larger payloads in research projects.
At the same time, our experienced and skilled technicians can accurately measure load during manual injection, effortlessly overcoming any technical challenges. Microinjection is also the most commonly used method in precision animal breeding research. By injecting gene-editing vectors into fertilized eggs, it is possible to make efficient modifications to the genome. Microinjection can also be used with CRISPRa and CRISPRi to transiently upregulate or downregulate specific genes in the mature cell genome.
Fig.1 Graphical Schematic of a Comparison between Setup and Time Necessary for the Microinjection vs. Electroporation of Embryos1,2.
Advantages of DNA Microinjection Solutions
DNA microinjection can be applied to embryos of farmed animals such as poultry, livestock, and aquatic species. Microinjection is the method of choice for delivering CRISPR/Cas machinery in precision animal breeding.
Only a small amount of payload is required, resulting in less stress on the mammalian embryo compared to virus- or liposome-mediated gene delivery solutions, reducing the potential for cell death.
Delivery of tool plasmids or proteins directly into embryos.
Does not require large quantities of specific reagents to open cell membranes.
Featured Services of DNA Microinjection Solutions
- Microinjection Solution Customization
BioVenic has developed microinjection solutions for farm animals, including poultry, livestock, and fish. Combined with CRISPR technology, we have the capability to offer comprehensive and tailored services for scientific research projects in the field of precision animal breeding.
- Processing of CRISPR Components
BioVenic offers proper digestion and purification of the payload, ensuring that only small doses are required for microinjection to achieve the editing target., which significantly enhance the overall editing efficiency and success rate.
- Gene Delivery Solutions Validation
In order to assess the effectiveness of the editing system, BioVenic conduct injection verification for each customized gene injection plan and culture the embryos until the gastrula stage for preliminary testing. The entire process is visualized through gene injection tracers, and the feasibility, safety, and reliability of the program are evaluated through multiple aspects, such as embryo survival rate and genome editing success rate.
Why Choose Us?
Safe and reliable gene delivery solution with high survival rate.
Experienced experimenters to ensure error-free execution of protocols.
Microinjection customization service dedicated to the gene editing for farmed animals.
Strict screening and verification, safe and efficient delivery of results.
BioVenic offers secure and direct microinjection services to facilitate gene delivery for researchers in the field of precision animal breeding worldwide. We have refined and optimized our service content and solutions at multiple levels with years of project accumulation. We are dedicated to addressing the challenges you encounter in the genetic transfer of farmed animals, providing you with perfect solutions. Please feel free to contact us to customize your personalized service plan.
Reference
- Lin, Jason C., and Alison L. Van Eenennaam. "Electroporation-mediated genome editing of livestock zygotes." Frontiers in genetics 12 (2021): 648482.
- Image retrieved from Figure 1 "Graphical schematic of a comparison between setup and time necessary for the microinjection vs. electroporation of embryos". Lin, Jason C., et al., 2021, used under CC BY 4.0, without any modification.