Calcium Phosphate-precipitated Gene Delivery in Precision Animal Breeding
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Animal Breeding and Genetics
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Therapeutic Protein and Peptide Development
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Animal Nutrition and Metabolism Solution
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Animal Nutrition and Metabolism Solution
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Calcium Phosphate-precipitated Gene Delivery in Precision Animal Breeding
Inquiry NowCalcium phosphate is one of the primary mineral constituents found in all calcified tissues of mammals. It is known for its biocompatibility, biodegradability, non-toxicity, and non-immunogenicity, which makes it an ideal and secure agent for intracellular delivery. BioVenic provides gene delivery vector customization services, which are alternatives to traditional viral vectors, due to their ability to carry a higher DNA load, lower immunogenicity and cytotoxicity, and their relative ease of production and amplification. BioVenic uses inorganic calcium to facilitate the transport of nucleic acid molecules by forming complexes with the nucleic acid backbone. The calcium phosphate precipitation we provide to customers enhances stability and facilitates targeted transport across the cell membrane by increasing its permeability.
Fig.1 Schematic Diagram of Several strategies via Calcium Phosphate-precipitated Gene Delivery1,2.
Services of Calcium Phosphate-precipitated Gene Delivery
DNA-calcium Phosphate Co-precipitation Particles Preparation
BioVenic prepares delivery nanoparticles by mixing the target delivery gene and inorganic calcium in specific proportions. We conduct property characterization to provide comprehensive customized services for customers in need of gene delivery services. We help address the technical challenges associated with gene delivery, relying on our advanced multi-level optimized calcium phosphate nanoparticle preparation technology.
In Vitro Calcium Phosphate-precipitated Gene Delivery
The technical application bottlenecks of calcium phosphate-precipitated gene delivery are limited transfection efficiency and sensitivity to the environment. BioVenic's gene delivery technology has been optimized and improved to meet the needs of effective transient or stable transfection for a variety of cell types. This allows for easy application of calcium phosphate precipitation gene delivery, ensuring safe and targeted gene loading.
Transfection Activity Assay
BioVenic has developed a specialized evaluation protocol for scientific researchers to assess the transfection efficiency of calcium phosphate-DNA co-precipitated nanoparticles. From aspects such as delivery efficiency, stability of the target gene, and impact on target cells, the feasibility and quality of the delivery plan are evaluated at multiple levels to ensure the successful implementation of the entire process.
DNA is mixed with inorganic calcium in a certain proportion and added to a phosphate buffer solution in a controlled manner.
The DNA-calcium phosphate co-precipitate adheres to the cell membrane and is transferred into the cell by endocytosis.
Calcium phosphate precipitated particles loaded with nucleic acidmolecules are obtained after incubation treatment.
Detect and verify the transfection effect of the target cells and whether the delivered genes can exist stably.
Why Choose Us?
- Optimized precipitation conditions, transfection conditions, and surfactant-added calcium phosphate co-precipitated nanoparticles.
- Customized calcium phosphate-precipitated gene delivery solution with stable transfection effect.
- Multi-level and multi-angle pre-verification of delivery results.
BioVenic offers customized gene delivery services that possess several appealing properties. These include a high capacity for nucleic acid adsorption, the ability of the transmitting plasmid to escape from the endosome, excellent biocompatibility, and bio-absorbability. This allows us to ensure that our customers' gene delivery is done effectively, accurately, and with minimal stress on the target cells. If you are seeking a secure and precise gene delivery solution for cultured animal cells in scientific research projects, please do not hesitate to contact us.
References
- Bakan, Feray. "Gene delivery by hydroxyapatite and calcium phosphate nanoparticles: a review of novel and recent applications." Hydroxyapatite-Advances in Composite Nanomaterials, Biomedical Applications and Its Technological Facets (2018): 157-176.
- Image retrieved from Bakan, Feray, 2018, used under CC BY 3.0. The original image was modified by changing title to "Schematic Diagram of Several strategies via Calcium Phosphate-precipitated Gene Delivery".