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Calcium Phosphate-precipitated Gene Delivery in Precision Animal Breeding
Calcium phosphate is one of the primary mineral constituents found in all calcified tissues of mammals. It is known for its biocompatibility, biodegradability, non-toxicity, and non-immunogenicity, which makes it an ideal and secure agent for intracellular delivery. BioVenic provides gene delivery vector customization services, which are alternatives to traditional viral vectors, due to their ability to carry a higher DNA load, lower immunogenicity and cytotoxicity, and their relative ease of production and amplification. BioVenic uses inorganic calcium to facilitate the transport of nucleic acid molecules by forming complexes with the nucleic acid backbone. The calcium phosphate precipitation we provide to customers enhances stability and facilitates targeted transport across the cell membrane by increasing its permeability.
Fig.1 Schematic Diagram of Several strategies via Calcium Phosphate-precipitated Gene Delivery1,2.
Services of Calcium Phosphate-precipitated Gene Delivery
DNA is mixed with inorganic calcium in a certain proportion and added to a phosphate buffer solution in a controlled manner.
The DNA-calcium phosphate co-precipitate adheres to the cell membrane and is transferred into the cell by endocytosis.
Calcium phosphate precipitated particles loaded with nucleic acidmolecules are obtained after incubation treatment.
Detect and verify the transfection effect of the target cells and whether the delivered genes can exist stably.
Why Choose Us?
- Optimized precipitation conditions, transfection conditions, and surfactant-added calcium phosphate co-precipitated nanoparticles.
- Customized calcium phosphate-precipitated gene delivery solution with stable transfection effect.
- Multi-level and multi-angle pre-verification of delivery results.
BioVenic offers customized gene delivery services that possess several appealing properties. These include a high capacity for nucleic acid adsorption, the ability of the transmitting plasmid to escape from the endosome, excellent biocompatibility, and bio-absorbability. This allows us to ensure that our customers' gene delivery is done effectively, accurately, and with minimal stress on the target cells. If you are seeking a secure and precise gene delivery solution for cultured animal cells in scientific research projects, please do not hesitate to contact us.
References
- Bakan, Feray. "Gene delivery by hydroxyapatite and calcium phosphate nanoparticles: a review of novel and recent applications." Hydroxyapatite-Advances in Composite Nanomaterials, Biomedical Applications and Its Technological Facets (2018): 157-176.
- Image retrieved from Bakan, Feray, 2018, used under CC BY 3.0. The original image was modified by changing title to "Schematic Diagram of Several strategies via Calcium Phosphate-precipitated Gene Delivery".