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Electroporation in Precision Animal Breeding
Electroporation has become an established physical method for delivering gene editing tools into cells. Electroporation delivery technology utilizes pulsed high-voltage current in a cell suspension buffer to rapidly create nanometer-sized pores in the cell membrane. This process enables the entry of fluid components with a diameter of tens of nanometers into the cell. BioVenic has mastered electroporation, a technique used to efficiently deliver gene-edited cargo to cell types that are traditionally difficult to manipulate. With our precise control of voltage and current application time, we effectively introduce various CRISPR delivery modes into host cells based on the specific research requirements of farmed animal breeding.
Fig.1 Workflow of the CRISPR-EZ Technology in Mouse Genome Editing1,2.
Electroporation-mediated Gene Editing Services
Application in Precision Animal Breeding
In the context of mammalian transgenics, electroporation has been used to transfer foreign DNA into cultured mammalian embryos at specific developmental stages. It has been reported that domestic animal blastocysts and fertilized eggs of fish, especially pigs and cattle, can be genetically manipulated through electroporation. Introduction of editing reagents at or shortly after fertilization can help reduce chimerism rates in species with long generation intervals. Studies in rodents suggest that electroporation can simplify the process of generating genetically modified livestock and make the technology more accessible to laboratories that lack expertise in microinjection (MI). It also needs to be mentioned that there have been limited studies conducted on cattle and pigs, which suggest that there is still a lot of work to be done in optimizing electroporation protocols. This optimization aims to improve editing and survival efficiency, as well as eliminate the generation of chimeric animals.
Conditions for Electroporation Delivery Protocol
| Conditions | Influence |
|---|---|
| Poring Pulse Voltage | Increasing the pore-forming voltage can increase the density of membrane pores and achieve a higher mutation rate, but the embryo viability decreases accordingly. |
| Pulses | The number and duration of pulses are proportional to the size and density of the stomata produced, as well as the amount of DNA entering the cell. |
| Concentration of Editing Reagents | The concentration of editing reagents affects the gene editing efficiency induced by electroporation, and optimizing the delivery concentration can greatly improve the editing efficiency. |
Why Choose Us?
Electroporation solutions more suitable for gene delivery in precision animal breeding.
Safe, non-toxic, and efficient delivery of results.
Advance research with ease using electroporation.
Highly customized scientific research services along with full professional and reliable technical support.
BioVenic has continuously optimized the technical conditions of electroporation and has launched electroporation services that are stable, accurate, and have a high survival rate. Our commitment is to facilitate the seamless application of electroporation technology, while ensuring the effective implementation of gene editing for researchers in the field of precision animal breeding worldwide. If you are seeking a gene delivery solution that is suitable for your research topic, please do not hesitate to contact us for further information regarding our custom electroporation services.
Reference
- Chen, Sean, et al. "Highly efficient mouse genome editing by CRISPR ribonucleoprotein electroporation of zygotes." Journal of Biological Chemistry 291.28 (2016): 14457-14467.
- Image retrieved from Figure 1 "CRISPR-EZ efficiently generates NHEJ-mediated indel mutations in mouse embryos". Chen, Sean, et al., 2016, used under CC BY 4.0. The original image was modified by extracting and using only part A and the title was changed to "Workflow of the CRISPR-EZ Technology in Mouse Genome Editing".