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Custom Animal Knock-in Cell Line
Animal gene knock-in refers to a gene editing technology that inserts exogenous genes into designated locations in the genome using various technical methods, allowing them to be stably expressed in cells. With years of experience in animal genome editing projects and a profound biological background, BioVenic can assist customers in inserting foreign gene fragments at the desired site and constructing stable animal knock-in cell lines. Our customized solutions fully consider the impact of the length of the knock-in gene fragment, knock-in efficiency, and variations in editing difficulty among different cell lines on the success rate. We tailor the technical process to optimize outcomes effectively. After multiple quality controls and repeated verifications, we guarantee the final delivery of stable animal cell lines with significant scientific value.
Fig.1 Schematic Diagram of the CRISPR/Cas9 Knock-in System.
Knock-in Design and Construction
BioVenic offers comprehensive services for designing and constructing gene editing systems for animal knock-in cell lines. We provide expert technical support and guidance, customizing precise gene knock-in solutions for your research projects. From guide RNA design and synthesis of homologous DNA fragments to predicting and evaluating editing efficiency, we are committed to offering customers a professional and dependable scientific research experience.
Animal Cell Transfection
BioVenic offers customers cell transfection technology services for animal cell lines, efficiently carrying out the co-transfection of gene editing systems and donor vectors into target cell lines. Our innovative RNP system reduces the technical obstacles associated with animal gene editing cell lines, minimizes immune responses, and enhances knock-in efficiency.
Enrichment and Single Cell Cloning
BioVenic assists customers in creating homologous cell lines following a successful knock-in. We have implemented a rigorous standardized workflow for enhancing positive cell lines and monoclonal screening services to minimize the influence of potential unstable factors during operations. By sequencing and screening the edited batch of transfected cell lines, we identify positive cell lines that have successfully knock-in the target gene. Subsequently, we conduct single cell cloning and amplify the positive cell lines to enhance the consistency and reliability of the results.
Quality Control and Validation
BioVenic launches dedicated services for quality control and validation of animal knock-in cell lines. We combined a second-generation sequencing platform with a molecular biology technology platform to sequence the insertion site region and determine if the cell line has achieved stable knock-in. BioVenic's experimental platform includes highly specific technical methods such as proteomics validation, immunology validation, and flow cytometry. These methods are used to conduct expression testing and analyze knock-in efficiency to ensure the stability and reliability of cell lines. We guarantee that the final delivered animal knock-in cell lines are sterile, free of mycoplasma, and have a normal chromosomal composition without lesions.
One-stop Animal Genome Editing
BioVenic utilizes the cutting-edge genome editing system to develop a precise animal genome editing technology platform. Compared with traditional ZENs and TALENs, we are advancing animal genetics and metabolism research in innovative and revolutionary ways to achieve practical goals related to various fields of animal cell biology and molecular biology. Our technical engineers and laboratory team have extensive project experience and a professional knowledge background. We are dedicated to offering high-quality services for site-specific knock-in of target DNA fragments and comprehensive design and synthesis of gene editing tools to create stable animal cell lines. Our goal is to assist you in utilizing the cas system to streamline the process of constructing animal knock-in cell lines and effectively address the constraints of animal genetic modification.
Workflow of Animal Knock-in Cell Line Development
Why Choose Us?
Perfect Service Process
Combining PCR and sequencing with subsequent verification ensures the accurate insertion of foreign genes. Regularly reporting experimental progress provides professional technical support.
High Editing Efficiency
The enhanced animal cell gene editing system and RNP system significantly improve the efficiency of homologous recombination during the creation of gene knock-in cell lines and decrease the off-target rate.
Rich Project Experience
With successful experience in constructing a variety of mammalian cells, we utilize various insertion methods, cloning vectors, and target cell lines based on customer project requirements to guarantee a high success rate for the project.
With years of project experience, BioVenic has become an expert in the fields of animal molecular biology and cell biology and continues to offer scientific researchers comprehensive and reliable customized solutions for establishing knock-in cell lines. We offer efficient one-stop services for designing and implementing animal genome editing solutions. Our services include cell line establishment, monoclonal screening, and cell line identification. At the same time, the technical team optimized key components such as gRNA and the cell transfection system to minimize the off-target rate and ensure the stability of the final cell line. After multiple rounds of quality control and a diverse portfolio of verification technologies, we are committed to ensuring high-quality services and delivering results of great scientific value. If you are considering incorporating animal knock-in cell line generation technology into your research project, please feel free to contact us to discuss more details.