Custom Animal Knockout Cell Line

Loss of key gene function is an important regulatory mechanism for studying metabolic activities in animals. Gene knockout in animal cells can eliminate the expression of the target gene, leading to a permanent loss of function. BioVenic has introduced a tailored one-stop solution for animal knockout stable cell lines, drawing on extensive project experience in the areas of cell biology and gene editing to effectively achieve the knockout of target genes. Our technical team is proficient in various gene editing methods, with expertise in the cutting-edge CRISPR/Cas9 system. This system combines the RNP delivery system with high editing efficiency and low toxicity to deliver editing elements directly into cells, enabling precise cutting of the target DNA sequence. Based on the specific needs of customers and the characteristics of target genes, we offer efficient and stable solutions for constructing animal knockout cell lines, enabling precise editing of genes in the animal genome.

Fig.1 Schematic Diagram of the CRISPR/Cas9 Knockout System.

CRISPR Protocol Design and Vector Construction

BioVenic has developed a comprehensive customized service solution for animal cell gene editing, which includes designing and developing gene knockout programs, synthesizing gRNA, and constructing editing vectors. Our mature animal genome editing platform has undergone multiple optimizations to reduce off-target rates and achieve target gene knockout at the desired site.

Animal Cell Transfection

BioVenic's animal cell transfection technology services ensure the efficient delivery and safe transfer of gene editing components into the necessary animal cells or tissues to achieve their editing effects. We offer personalized services for researchers developing animal knockout cell lines. We focus on enhancing and optimizing crucial elements like carrying capacity, immunogenicity, and delivery effectiveness of the transfection system to guarantee that customers receive top-notch, tailored services.

Enrichment and Single Cell Cloning

BioVenic completes the construction of homologous knockout cell lines for customers after successful editing. We have established strict standardized work processes to reduce errors caused by operations. By sequencing and screening the edited batch-transfected cell library, the successfully edited positives are obtained. When assessing editing efficiency, the positive cells are single-cell cloned and amplified to establish a stable knockout cell line.

Quality Control and Validation

BioVenic has introduced a comprehensive one-stop service for quality control and verification of animal knockout cell lines. We conducted an initial basic verification to determine if the cell lines had achieved complete biallelic knockout by sequencing the target loci. BioVenic's experimental platform includes highly specific technical methods like proteomics verification, immunology verification, and flow cytometry. These methods are used to conduct expression testing and off-target analysis, ensuring the stability and reliability of cell lines.

One-stop Animal Genome Editing

BioVenic utilizes cutting-edge precision animal genome editing technology based on the cas 9 system. We are advancing animal genetic and metabolic research in innovative and revolutionary ways to achieve various goals, such as enhancing the traits of farmed animals to boost productivity, health, and welfare. Our technology engineers have extensive practical experience and a professional knowledge background. We are dedicated to offering high-quality genome knockout services and comprehensive design and synthesis of gene editing tools for establishing stable animal cell lines. Our goal is to assist you in leveraging the power of the genome editing system to decrease the workload of genome knockout and acquire dependable and consistent target animal cell lines.

Workflow of Animal Knockout Cell Line Development

Why Choose Us?

As an expert in animal molecular biology and cell biology, BioVenic provides scientific researchers with comprehensive and reliable customized solutions for establishing knockout cell lines. Whether it is for small fragment knockout, frameshift mutation knockout, or large fragment knockout, we design efficient genome editing plans for you. At the same time, the technical team optimized key components such as gRNA and experimental operations to minimize the off-target rate and ensure simultaneous deletion of alleles in the final cell line. After multiple rounds of quality control and a diversified combination of verification technologies, we are committed to ensuring high-quality services and delivering results of great scientific value. If you are experiencing challenges in creating animal knockout cell lines, please do not hesitate to contact us for access to unique experimental solutions.