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Custom Animal Overexpression Cell Line
Gene overexpression is one of the common methods used to study gene function. By increasing the expression level of a target gene in cells, we can gain a better understanding of the gene's function and regulatory mechanisms. BioVenic offers personalized services for creating animal overexpression cell lines. With extensive experience, we specialize in achieving gene overexpression in a wide range of cell types. Through advanced viral packaging methods and transfection techniques, BioVenic has established numerous stable cell lines that overexpress the target genes.
Gene Synthesis
Gene synthesis is a crucial step in establishing animal cell lines that consistently express a specific gene. BioVenic offers high-quality gene synthesis services utilizing host cell information and gene sequences provided by our customers. We can efficiently deliver challenging sequences that contain repetitive elements, high GC content, hairpin structures, and consecutive single bases.
Vector Construction
Vector construction ensures that the target gene is efficiently expressed and functions in the host cell. BioVenic offers custom vector construction services on demand. We offer a diverse range of vector systems optimized for various research applications, validated both in vitro and in vivo. Utilizing our service for vector customization and cloning will significantly reduce the time and enhance the efficiency of vector construction.
| Sequence Optimization |
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| Insertion of Specific Sequences |
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Transfection/Transduction
BioVenic provides transduction and transfection services. We use advanced transfection techniques to introduce target genes into specific cells. For challenging cell types, we utilize special viral vectors or optimized transfection conditions.
Lentiviral Packaging
Lentiviral vectors are increasingly favored for their ability to introduce specific genes into hard-to-transfect cells, including primary and stem cells, as well as non-dividing cells. This capability significantly boosts the success rate of gene transduction. Additionally, lentiviruses offer clear advantages in transient transfection processes and the establishment of stable cell strains. BioVenic offers lentiviral packaging services, providing customers with a variety of options for titer and specifications.
Positive Cell Clone Screening
BioVenic provides services for screening positive cell clones to achieve genetically and phenotypically consistent single-cell clones from transduced cell populations. This is done through antibiotic screening and monoclonal isolation techniques. Cells are exposed to a medium containing a specific antibiotic, and only those containing the resistance gene survive, enabling initial screening. After screening, the cells are separated into individual cells using the limited dilution method or flow cytometric sorting. Each single cell is subsequently cultured in separate wells to form the monoclonal cell population desired by the customer.
Relative Development Services
BioVenic offers services related to screening and validating overexpressing cell lines. This involves verifying gene expression levels and protein function using FACS, Western blot, and PCR. We also offer cell cryopreservation services for our customers. Successfully validated overexpression cell lines will be expanded and properly frozen to ensure a stable long-term supply and reuse.
Flow Chart of Custom Animal Overexpression Cell Line Development
Why Choose Us?
BioVenic can achieve gene overexpression in a wide range of cell types, including some that are difficult to transfect. Whether it is a conventional cell line or specialized primary cells, we can provide solutions.
By utilizing advanced transfection and viral packaging technologies, we can accurately integrate target genes into highly active sites in the genome. These sites are selected to ensure not only high gene expression but also stability and safety of expression.
According to the specific needs of customers, we can customize our service solutions. For your special research needs, our team can provide professional consultation and technical support to ensure the realization of your experimental goals.
BioVenic offers custom animal overexpression cell line services, meeting veterinary research needs for animal-specific gene expression studies. We provide a one-stop solution from gene synthesis, vector construction, and viral packaging, to cell transduction and monoclonal screening, utilizing state-of-the-art genetic engineering technologies. If you are seeking a research partner to develop overexpression cell lines, please do not hesitate to contact us.