Custom Animal Knockdown Cell Line

Animal knockdown cell lines are widely used in various molecular biology studies, including gene function analysis, animal disease model development, veterinary drug development, and target verification. RNA interference (RNAi) is a gene silencing phenomenon induced by double-stranded RNA (dsRNA). Its mechanism involves inhibiting gene expression by interfering with the transcription or translation of specific genes. To achieve the knockdown of various target genes in cells from different sources for scientific research purposes, BioVenic has introduced a comprehensive solution for designing and constructing animal knockdown cell lines. We have mastered a variety of transfection methods to deliver short hairpin RNA (shRNA) molecules into cells to form small interfering RNA (siRNA), which can stably inhibit gene expression for an extended period and knock down target mRNA. Our rigorous quality control and screening system further ensures the acquisition of positive and stable cell lines for gene knockdown, along with related data and reports.

Fig.1 Biological Principle of RNAi Interference to Silence Target Genes. ^1,2

shRNA Design and Vector Construction

BioVenic is proficient in short hairpin RNA (shRNA) design, synthesis, and vector construction technology. By stably integrating into the genome of animal target cell lines, the gene silencing can be inherited by progeny cells without the need to transfect cells multiple times. Our expert team guides the design and synthesis process of shRNA and optimizes its structure to ensure efficient RNAi knockdown.

shRNA Delivery

BioVenic induces RNA interference (RNAi) by utilizing short hairpin RNA (shRNA) expressed from plasmid vectors or virally mediated integrated genomic DNA. For the delivery of shRNA, we offer two technical services: lipofection of plasmid vectors and transduction delivery of viral particles to ensure stable expression of shRNA in the nucleus. We have developed a range of exogenous gene delivery systems for mammalian cells to effectively cater to the personalized requirements of our customers.

Stable Cell Line Generation

BioVenic conducts high-throughput screening of the cell line population that has been transfected with the knockdown system, amplifies and further screens the positive screening results, verifies the cell lines showing the best knockdown efficiency, and delivers them.

Quality Control and Validation

BioVenic has a comprehensive quality inspection process for animal stable cell line products to ensure that the final delivered cell lines pass a series of standard quality control tests, including sterility testing and standard QC testing. We are committed to providing customers with multiple verified cell lines that exhibit the best knockdown efficiency and the most stable effects.

RNA Interference (RNAi) Technology

RNA interference (RNAi) technology services utilize RNA molecules to inhibit gene expression and achieve knockdown of target genes in animal genomes. Inactivation or degradation of target mRNA can lead to downregulation or silencing of the target gene's expression. BioVenic has developed an RNAi technology service for various scientific research projects, including gene function exploration, veterinary drug development, and research on animal disease metabolism. Compared with transient silencing, achieving permanent knockdown can be done by stably integrating shRNA into the cell genome, eliminating the need to transfect cells multiple times. BioVenic's shRNA synthesis and design services exclusively focus on viral and plasmid shRNA constructs for the entire animal genome. We utilize structurally optimized shRNA designs of varying lengths to create shRNA expression constructs with high knockdown efficiency and minimal off-target effects. Our RNAi technology efficiently supports customers in completing shRNA design and synthesis, vector construction, transfection, mRNA expression, and downstream protein expression verification services.

Workflow of Animal Knockdown Cell Line Development

Why Choose Us?

BioVenic offers a professional and systematic technical platform for constructing stable animal cell lines, providing shRNA gene silencing services for scientific researchers. Our scientists have extensive experience using shRNA to achieve knockdown in stable cell lines. From designing and synthesizing the target gene shRNA to constructing vectors, transfecting/transducing target cells, and screening to obtain stable cell lines with high knockdown efficiency, we offer a comprehensive one-stop solution. Our knockdown stable cell lines have undergone rigorous testing and validation, resulting in a stable genetic knockdown. If you are utilizing RNAi to characterize biological pathways or enhance your comprehension of the molecular mechanisms underlying animal disease states, please do not hesitate to contact us to discuss your exclusive animal knockdown cell line needs.

References

1. Image retrieved from Figure 1 "Types of RNAi constructs introduced into target cells for silencing." Perwitasari et al., 2013, used under [CC BY 3.0] (https://creativecommons.org/licenses/by/3.0/). The original title was changed to "Biological Principle of RNAi Interference to Silence Target Genes."
2. Perwitasari, Olivia, et al. "siRNA genome screening approaches to therapeutic drug repositioning." Pharmaceuticals 6.2 (2013): 124-160.