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Veterinary Bacterial Pathogen Proteomics and Metabolomics Services
Advanced technologies like proteomics and metabolomics are now being employed to gain comprehensive insights into bacterial mechanisms, aiding in the diagnosis, treatment, and prevention of diseases in animals. BioVenic's veterinary bacterial proteomics and metabolomics services offer state-of-the-art solutions to meet these needs, providing in-depth analyses that contribute to the health and well-being of animals.
Veterinary Bacterial Proteomics Services
In the context of veterinary science, bacterial proteomics focuses on identifying and quantifying the proteins expressed by bacterial pathogens that affect animals. This includes understanding protein modifications, interactions, and the roles they play in bacterial virulence and antibiotic resistance. BioVenic offers comprehensive proteomics services with our advanced technology platforms.
- Veterinary Bacterial Proteomics Platforms
Mass Spectrometry (MS)
- High-resolution MS for protein identification and quantification.
- Tandem MS (MS/MS) for detailed peptide sequencing.
- Label-free quantification and isotope labeling techniques (such as SILAC).
Two-Dimensional Gel Electrophoresis (2-DE)
- Separation of complex protein mixtures.
- Comparative analysis of protein expression under different conditions.
Protein Microarrays
- High-throughput analysis of protein interactions.
- Profiling of bacterial protein expressions in various environments.
Fig.1 Hierarchical clustering.1
Veterinary Bacterial Metabolomics Services
In veterinary science, bacterial metabolomics focuses on understanding the metabolic profiles of bacterial pathogens, which can reveal crucial information about their pathogenicity, survival mechanisms, and interactions with host organisms. BioVenic has established several metabolomics technology platforms to support veterinary researchers.
- Veterinary Bacterial Metabolomics Platforms
Mass Spectrometry (MS)
- Gas Chromatography-MS (GC-MS) for volatile metabolites.
- Liquid Chromatography-MS (LC-MS) for a broad range of metabolites.
Capillary Electrophoresis-MS (CE-MS)
- High-resolution separation of ionic metabolites.
- Suitable for small volume samples.
Nuclear Magnetic Resonance (NMR) Spectroscopy
- Non-destructive analysis of metabolic profiles.
- High reproducibility and quantitative accuracy.
Service Workflow
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Sample Collection and Preparation
Collection and preparation of veterinary bacterial samples from clinical or environmental sources. Samples undergo preparation, including homogenization, lysis, and protein extraction for proteomics, or metabolite extraction for metabolomics. -
Analytical Techniques
- Proteomics: Utilizes suitable technology platforms for protein identification and quantification.
- Metabolomics: Employs MS or nuclear magnetic resonance (NMR) for metabolite profiling.
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Data Analysis
Advanced instruments acquire high-throughput data, generating spectra for analysis. We utilize bioinformatics tools and software analyze data, identifying proteins, peptides, and metabolites, and quantifying their abundance. Results are interpreted in the context of the veterinary issue, providing insights into bacterial functions and interactions. -
Reporting and Interpretation
Findings are compiled into comprehensive reports for veterinary researchers, highlighting key biomarkers and potential therapeutic targets, customized for your research requirements. Follow-up consultations offer further insights and recommendations based on the findings.
Applications
Applications of veterinary bacterial proteomics and metabolomics are extensive and impactful.
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BioVenic's veterinary bacterial proteomics and metabolomics services are designed to provide comprehensive insights into bacterial pathogens affecting animals. With our expertise, advanced technology, and commitment to quality, we are your ideal partner in veterinary bacterial research and diagnostics. Please don't hesitate to contact us to learn more about how our services can benefit your work and contribute to the health and well-being of animals.
Reference
- Dupré, Mathieu, et al. "Optimization of a top-down proteomics platform for closely related pathogenic bacterial discrimination." Journal of Proteome Research 20.1 (2020): 202-211.