Veterinary Bacterial Pathogen Transcriptomics Services

In the rapidly evolving field of veterinary research, understanding the complex interactions between bacterial pathogens and their animal hosts is crucial. BioVenic offers veterinary bacterial pathogen transcriptomics services with cutting-edge approach to uncovering these interactions at the molecular level. By leveraging advanced RNA sequencing (RNA-seq) techniques, our services provide comprehensive insights into gene expression, functional genomics, and the dynamics of bacterial pathogens within their animal hosts.

Background

BioVenic's transcriptomics services utilize RNA-seq and its derivative approaches to study the transcriptomes of bacterial pathogens infecting animals. A transcriptome represents the complete set of RNA transcripts produced by the genome under specific circumstances or in a specific cell. By analyzing these transcripts, researchers can gain detailed information about gene expression patterns, gene organization, and other functional genomic features of bacterial pathogens. The primary goal of our services is to provide a deep understanding of how bacterial pathogens interact with their animal hosts. This includes identifying genes that are actively expressed during infection, understanding the mechanisms of antibiotic resistance, and exploring the immune responses of animal hosts to bacterial invasion. Such insights are invaluable for developing new diagnostic tools, therapeutic strategies, and vaccines to combat bacterial infections in animals.

Veterinary Bacterial Pathogen Transcriptomics Service Workflow

• Sample Collection and Preparation

  

  The process begins with the collection of samples from infected animals. These samples can include tissues, fluids, or bacterial cultures. Proper sample handling and preservation are critical to maintaining RNA integrity.

• RNA Extraction

  

  High-quality RNA is extracted from the collected samples. This step involves isolating total RNA, including messenger RNA (mRNA), which is crucial for subsequent sequencing.

• RNA Sequencing (RNA-seq)

  

  The extracted RNA is then subjected to RNA sequencing. This involves converting RNA into complementary DNA (cDNA) libraries, which are sequenced using high-throughput sequencing technologies. The resulting sequence data provides a comprehensive view of the bacterial transcriptome.

• Data Analysis

  

  Advanced bioinformatics tools are used to analyze the sequencing data. This includes aligning the reads to reference genomes, quantifying gene expression levels, and identifying differentially expressed genes. Functional annotation and pathway analysis further elucidate the biological significance of the findings.

• Reporting and Interpretation

  

  The final step involves compiling the results into detailed reports. These reports include comprehensive analyses of gene expression profiles, potential antibiotic resistance genes, and insights into animal host-pathogen interactions.

Fig.1 Display of RNA-seq data.¹

Applications

BioVenic's veterinary bacterial pathogen transcriptomics services offer a multitude of advantages for veterinary research. Our service provides comprehensive insights into gene expression, capturing the intricate responses of bacterial pathogens to diverse conditions. With high-throughput sequencing technologies, our services ensure unparalleled precision and accuracy in measuring gene expression levels, guaranteeing reliable data for further analysis. Transcriptomics uncovers novel genes and pathways crucial for bacterial survival and pathogenicity, serving as potential targets for developing new antibiotics and therapeutics. The dynamic nature of RNA-seq enables real-time monitoring of gene expression changes, offering immediate insights into pathogen adaptation. Our services are customizable to specific research needs, focusing on particular pathogens, animal host interactions, or environmental influences. For your veterinary bacterial pathogen project, please don't hesitate to contact us to get a quote tailored to your requirements.

Reference

1. Croucher, Nicholas J., and Nicholas R. Thomson. "Studying bacterial transcriptomes using RNA-seq." Current opinion in microbiology 13.5 (2010): 619-624.