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Veterinary Antigen Expression
Numerous expression methods are available to produce antigens for veterinary vaccine development based on specific research and development requirements. BioVenic has established several antigen expression systems suitable for various types of antigens, considering the biochemical nature of antigens, development scale, and the intended application, which ensure accurate expression of antigen components for veterinary vaccine development, thereby enhancing the vaccine's immunogenicity.
Our Services
Protein Antigen Expression
BioVenic's veterinary protein antigen expression service offers an effective and reliable approach to accelerate your vaccine development projects.
- Stable Cell Line Development
Stable cell lines refer to cells that continuously and stably express specific genes or interfere with specific gene expression. Due to their high stability, they are widely used in recombinant protein production. BioVenic has established a high-yield and stable cell line development platform. We ensure comprehensive cell line quality analysis, including physicochemical properties, gene stability, and expression stability.
- Expression Systems
BioVenic has established a range of host systems to provide recombinant protein expression services, allowing you to obtain protein antigens suitable for immunizing against various pathogens.
| Expression Systems | Description |
|---|---|
| Bacterial Expression Systems | Fast and cost-effective. |
| Yeast Expression Systems | The most economical eukaryotic expression system, suitable for both secretion and intracellular expression. |
| Baculovirus-Insect Cell Expression Systems |
Easy to scale up from the laboratory to large-scale production systems. Compared to bacterial expression systems, it offers higher solubility, post-translational modification capabilities, and protein secretion yield. |
| Mammalian Expression Systems | The best choice for producing mammalian proteins with native conformation and post-translational modifications, and they can secrete properly folded glycoproteins. |
Peptide Antigen Expression
Peptide antigens represent individual immunodominant epitopes from various viruses, bacteria, and other species of pathogens. BioVenic has established a synthetic platform for generating antigenic peptides representing specific epitopes for use in veterinary vaccine development, aimed at activating protective immune responses in animal post-vaccination.
| Peptide Synthesis | ||
|---|---|---|
| Peptide sequence | Peptide purity | Peptide specifications |
| Peptide Modification | |
|---|---|
| Branched peptides. | Cyclic peptides. |
| N- and C-terminal modifications. | Phosphorylation modifications. |
| Methylation modifications. | Carrier proteins (KLH, BSA, and OVA). |
Nucleic Acid Antigen Expression
Veterinary DNA or mRNA vaccines are not directly involved in protein expression, but use nucleic acids to encode antigens. Animals undergo protein expression in the body (through transcription and translation) to elicit a protective immune response after vaccination.
- Plasmid DNA Preparation
BioVenic offers plasmid DNA preparation as a key service in veterinary vaccine development. Our dedicated professionals undertake the synthesis of target genes, cloning of DNA into plasmid vectors, amplification within bacterial cultures, and subsequent purification for use in the development of veterinary vaccines.
- mRNA In Vitro Transcription and Capping
This service is pivotal in the design of mRNA vaccines, encompassing the synthesis of functional mRNA from plasmid DNA templates through in vitro transcription. Our professionals advance the process by linearizing plasmid DNA, performing in vitro transcription of mRNA using a cell-free system, and capping the mRNA using cap analogs or virus-encoded capping complexes, following the service workflow.
Fig.1 Production process of mRNA. 1
Why Choose Us?
A comprehensive veterinary antigen platform, covering services from gene synthesis to protein characterization, expression, and purification.
Diverse expression systems available to choose from based on development and cost requirements.
Our expression services undergo strict quality control.
Antigens, being a vital component of veterinary vaccines, need to be efficiently expressed to stimulate the appropriate protective immune response in the host organism. BioVenic has established expression platforms for different antigen types to assist veterinary vaccine development activities. If you need antigen expression services in veterinary vaccine development, please contact us. We provide you with dedicated and satisfactory services.
Reference
- Versteeg, Leroy, et al. "Enlisting the mRNA vaccine platform to combat parasitic infections." Vaccines 7.4 (2019): 122.