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Veterinary Antigen Characterization
As a crucial aspect of veterinary vaccine development, antigen characterization is primarily used to investigate the properties, structure, and immunogenicity of potential vaccine antigens. It aims to elucidate structure-function relationships, study the mechanisms of antigen action, and determine antibody-antigen binding interactions. BioVenic offers antigen characterization services with a variety of methods to meet your diverse needs, ensuring a satisfactory start to veterinary vaccine research and development.
Our Services
BioVenic employs a comprehensive range of services to assess the characterization of components such as proteins, glycoproteins, DNA, RNA, carbohydrates, and others, serving as antigens. From the perspective of antigen structure to antigen-antibody interactions, BioVenic's services encompass various test parameters that are readily available for utilization.
Sequencing
- Nucleic Acid Sequencing
Determining the nucleotide sequences of pathogen DNA and RNA, including their base composition and sequence length, helps identify whether the sequence contains genes or coding regions that can be used to activate protective immune responses in animals.
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- Protein Sequencing
For protein or peptide antigens, the primary structure is the linear amino acid sequence that makes up the polypeptide chain. Information obtained through protein sequencing can be used for synthesizing peptide segments used as immunogens, among other purposes.
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Structure Determination
- Protein Structure Determination
Protein structure refers to the three-dimensional arrangement of amino acid atoms in a protein molecule, which folds into one or more spatial conformations to realize biological functions. In order to understand antigens at the molecular level, it is important to determine their three-dimensional structure.
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- Glycoprotein Structure Determination
Glycoprotein structure characterization is a complex task because of the greater diversity and heterogeneity of glycoproteins compared to the amino acid sequences of proteins. By mass spectrometry, it is possible to determine the structure of the glycan moieties in glycoproteins, including the composition of the glycan chains and the way they are connected.
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Modification
To enhance the stability and immunogenicity of veterinary vaccine antigens, modifications including post-translational modifications of proteins and methylation or phosphorylation of nucleic acids are carried out according to the development requirements of veterinary vaccines. The nature of antigen modifications varies based on the specific vaccine antigen and its intended applications. BioVenic provides the following characterization methods to determine the structural status of veterinary vaccine antigens after the completion of modifications.
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Purification
No matter what type of antigen, isolating the desired antigenic components and removing unwanted residual components is one of the most labor-intensive steps in the antigen extraction process. It is not an overlooked stage, as antigen purity is a critical indicator of success in downstream applications of veterinary vaccines. BioVenic has established these technology platforms to assess antigen purity and to ensure that extracted antigenic components meet the quality standards required for veterinary vaccine development.
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Antigen-Antibody Interaction
For intact animal vaccine antigens such as bacteria and viruses, BioVenic provides serological experiments for the visual observation of interactions between antigens and antibodies.
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Fig.1 Response flow of the hemagglutination inhibition test. 1
Epitope mapping is the essential procedure for pinpointing the specific binding sites between a target antigen and antibodies. Several techniques are employed by BioVenic for this purpose.
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Veterinary Pathogenic Antigen Discovery Solutions
BioVenic provides one-stop veterinary vaccine development solutions for the identification and characterization of antigens from different types of both animal-specific and zoonotic pathogens.
Veterinary Virus Identification and Characterization
Veterinary Bacterial Pathogen Identification and Characterization
Veterinary Fungal Pathogen Identification and Characterization
Why Choose Us?
Widely applicable, capable of characterizing a variety of veterinary vaccine antigen.
The platform established by BioVenic offers high sensitivity, allowing even weak antigen-antibody interactions to be characterized.
Characterization results demonstrate good repeatability.
Relatively short project timelines show high efficiency.
Various candidate veterinary vaccines are being developed to prevent infectious diseases in animals and safeguard animal health. These products are based on a wide range of technologies, posing challenges in antigen characterization and veterinary vaccine development. BioVenic's vaccine development support team provides a comprehensive suite of antigen characterization services to select excellent vaccine antigens. Contact us now for an in-depth discussion about veterinary antigen characterization services.
Reference
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From CDC:
https://www.cdc.gov/flu/images/professionals/laboratory/hi-assay-components.jpg?_=41449