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Feed Prebiotics-Fructo-Oligosaccharides (FOS)

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Production and Characterization In Vitro Study In Vivo Evaluation Mechanism Study Prebiotics Development Applications Development Workflow Why Choose Us?

Fructo-oligosaccharides (FOS) or oligofructose are a type of fructans, consisting of a linear chain of fructose units linked by β (2→1) bonds. Typically, FOS contains glucose units at the ends of the chain, also linked by β (2→1) bonds. The degree of polymerization (DP) of FOS is usually less than 10. BioVenic offers a comprehensive range of solutions for the development of FOS prebiotics for animal feed. These solutions encompass production optimization, characterization, evaluation of in vivo and in vitro effects, mechanism studies, and the development of finished products.

Fig. 1 Fructo-oligosaccharides (FOS) (Ibrahim, 2018)Fig. 1 Fructo-oligosaccharides (FOS)1

Production and Characterization of FOS Prebiotics

  • Production of FOS Prebiotics

There are two methods for the industrial production of FOS, enzymatic or inulin hydrolysis. Our solutions focus on developing more cost-effective production methods, such as utilizing agricultural waste, developing microbial strains, and producing FOS-producing enzymes.

  • Characterization of FOS Prebiotics

Our technology platforms offer various techniques to characterize the structure, purity, composition, and function of FOS. These include methylation analysis, DPPH assay, GC-MS, NMR, IR, and more.

In Vitro Study of FOS as Animal Feed Prebiotics

  • In Vitro Prebiotic Efficacy of Prebiotic FOS

By studying the effects of FOS on the growth of probiotics, such as lactic acid bacteria, we facilitate research on the in vitro probiotic effects of FOS. We also help determine the appropriate dosage of additives and investigate whether FOS inhibits the growth of harmful bacteria.

  • In Vitro Digestion of Prebiotic FOS

The resistance of FOS to gastric juice and various enzymes in the gastrointestinal system is an essential characteristic of prebiotics. Our in vitro digestion models assist in studying the gastrointestinal stability of FOS by simulating digestion with gastric, salivary, pancreatic, and brush border enzymes

  • In Vitro Fermentation of Prebiotic FOS

Using fecal or digesta microorganisms as inoculum and FOS as substrate, our in vitro fermentation models help analyze the impact of FOS on the microbiota of the animal digestive tract. Through technologies such as microbiota analysis, fermentation product analysis, and sugar analysis, we obtain valuable insights into the fermentation characteristics of FOS.

In Vivo Evaluation of FOS in Animals

We offer a range of in vivo models for farm animals and companion animals. By combining feed analysis, animal physiology, biochemistry index analysis, animal metabolite analysis, and gut microbial and metabolite analysis, we can assess the effects of FOS from different sources and dosages. Key indicators include growth performance, intestinal health, intestinal microbial composition, structure, product quality, and immune response.

Mechanism Study of Feed Prebiotics FOS

Our solutions enable the investigation of how prebiotics like FOS impact animals through their effects on gastrointestinal microbes and microbial metabolites. We provide animal nutrition and metabolism services that encompass various studies, including high-throughput microbiome, metabolomics, proteomics, and other omics technologies. These studies aid in the development of assays to better understand the mode of action of FOS.

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Feed FOS Prebiotics Development

Our one-stop solution offers development and performance measurement services for FOS probiotic products. Our services provide valuable information for the development of FOS probiotic products, confirm their effects in animal diets, and determine appropriate dosages.

Applications of FOS as Feed Prebiotics

FOS has numerous benefits in animal feed. It promotes the growth of beneficial bacteria, inhibits harmful bacteria, improves nutrient digestion and absorption, enhances the microecological environment, and boosts immunity and production performance in livestock and poultry. See Table 1 for specific application effects of FOS in various animal types.

Table. 1 Application effects of FOS in animal feed

Animal types Effects
Pigs
  • Helps maintain a healthy GIT environment in weanling pigs by promoting colonization of Bifidobacteria or reducing E. coli.
  • Increases growth performance and digestibility of growing pigs.
  • Increases antioxidant ability of piglets.
Chickens
  • Enhances antioxidant activity and cellular immune response in broiler chickens.
  • Increases laying rate, average egg weight, feed egg ratio, fertility rate, etc., in hens.
  • Reduces cholesterol and triglyceride levels, and improves nutrient metabolizability in hens.
Cattle
  • Affects rumen fluid bacterial flora and improves stability through the supplementation of functional oligosaccharides (including FOS).
  • Enhances growth of nursing dairy calves and stimulates the persistence of Bifidobacterium and hindgut microbiome maturation.
Companion animals
  • Improves gut health in dogs by altering microbial ecology in the ileum and feces through supplementation of FOS plus MOS.
  • Modifies the fecal flora in cats when added to their diet.

Development Workflow of Feed Prebiotics FOS

Fig. 2 Development workflow of feed prebiotics FOS (BioVenic Original)

Why Choose Us?

One-stop Technology Platform

Our integrated technology platform covers the entire development process of FOS as animal feed prebiotics, providing comprehensive solutions.

Multiple Animal
Models

We offer various in vivo and in vitro animal models to study the effects of FOS prebiotics, enabling a deeper understanding of their impact.

Characterization Technologies

With a wide range of characterization techniques, we can thoroughly analyze the characteristics of FOS, including structure, purity, antioxidant properties, and more.

Our feed prebiotic FOS development solutions focus specifically on animal feed applications. BioVenic provides professional and customized services for FOS production, characterization, in vitro testing, in vivo evaluation, mechanism studies, and finished product development. If you are interested in our solutions, please contact us with your research plan, and we will be pleased to assist you with the appropriate research services.

Reference

  1. Ibrahim, Osama O. "Functional oligosaccharides: Chemicals structure, manufacturing, health benefits, applications and regulations." J Food Chem Nanotechnol 4.4 (2018): 65-76. Under Open Access license CC BY 4.0, without modification.
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