Feed Prebiotics-Galacto-Oligosaccharides (GOS)

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Feed Prebiotics-Galacto-Oligosaccharides (GOS)

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Production and Characterization In Vitro Study In Vivo Evaluation Mechanism Study Prebiotics Development Applications Development Workflow Why Choose Us?

The health advantages of Galacto-oligosaccharides (GOS) as prebiotics involve stimulating the growth of beneficial bacteria, such as Bifidobacterium and Lactobacillus species, while inhibiting the growth of harmful bacteria like Escherichia coli, Salmonella typhimurium, and Clostridium species. This is accomplished through competitive exclusion. BioVenic offers comprehensive solutions for the development of GOS feed prebiotics specifically designed for animal nutrition researchers. Our solutions cover various aspects, including production and characterization, in vivo and in vitro evaluations, mechanism studies, and finished product development for feed prebiotics GOS.

Production and Characterization of GOS Prebiotics

Production of GOS Prebiotics

GOS naturally occur in milk but at low concentrations. They are commercially produced through enzymatic trans galactosylation of lactose, a milk sugar substrate, using microbial enzyme beta-galactosidase. Our GOS prebiotics production solution assists in the discovery of novel microorganisms and microbial enzymes for GOS production. By optimizing substrate, conditions, and enzymes, we enhance the production process to achieve higher GOS yield.

Characterization of GOS Prebiotics

GOS consists of various di-, tri-, tetra-, and pentasaccharides. Characterizing different GOS types involves fractionation through yeast treatment, size-exclusion chromatography, or hydrophilic interaction liquid chromatography, followed by analysis using methods like methylation analysis, GC-MS, NMR spectroscopy, HPAE-PAD-MS, and ESI-MS. Through a combination of analytical techniques, we aid researchers in exploring the characteristics of GOS prebiotics.

In Vitro Study of GOS as Animal Feed Prebiotics

In Vitro Prebiotic Efficacy of GOS

Evaluating the prebiotic efficacy of GOS involves studying the selective consumption by bifidobacteria and lactobacilli, which contribute to the prophylactic health effects associated with GOS. Our solution enables the assessment of prebiotic GOS's effects on specific probiotics through in vitro studies, facilitating synbiotic development. In addition, our in vitro cell models provide insight into the mode of action of GOS as a prebiotic.

In Vitro Digestion of Prebiotic GOS

We conduct in vitro digestion experiments of GOS using simulated saliva, gastric, and intestinal digestion models. These experiments unveil information about the digestion characteristics of GOS and serve as a reference for in vivo animal experiments and subsequent product development.

In Vitro Fermentation of Prebiotic GOS

By introducing GOS into in vitro fermentation models, we investigate its impact on fermentation microbiota, microbial metabolites, gas production, and more. This aids animal nutrition researchers in determining the prebiotic effect of GOS under specific in vitro fermentation conditions and discovering its potential for synbiotic combinations with specific probiotics.

In Vivo Evaluation of GOS in Animals

Our in vivo animal models enable researchers to examine the effects of GOS as a feed additive in various animal species. We provide comprehensive analysis solutions, including feed analysis, animal nutrition and metabolism analysis, analysis of the digestive tract microbiome and metabolome, and a range of animal nutrition and metabolism research solutions. These analyses yield valuable data for studying animal immunity, digestion and metabolism, growth performance, product quality, and more.

Mechanism Study of Feed Prebiotics GOS

Prebiotics' health benefits for the host involve various mechanisms, such as selectively stimulating the growth of beneficial probiotic organisms, modulating cytokines to inhibit the secretion of pro-inflammatory markers, releasing SCFAs, and enhancing mineral absorption. Our integrated omics platform allows for the discovery of biomarkers (microbes, metabolites, proteins, genes, etc.) involved in the mode of action of prebiotic GOS, aiding in mechanism studies.

Feed GOS Prebiotics Development

We assist animal nutrition researchers in the development and validation of GOS feed prebiotic products. Through our in vivo and in vitro evaluation tools, we determine the appropriate GOS dosage in different animal feeds, investigate the effects of different GOS forms, and provide references for subsequent applications. Additionally, our analytical tools allow for the assessment of finished product quality.

Applications of GOS as Feed Prebiotics

GOS applied in animal feed offer benefits such as improved health, growth, gut microbial ecology, reduced antibiotic usage, prevention of early mortality, and diminished fecal odor. GOS has also been effective in suppressing methane production in ruminants. Table. 1 provides an overview of the application effects of GOS in different animal feed types.

Table. 1 Application effects of GOS in animal feed

Animal Types	Effects
Pigs	Improve barrier function and relieves colonic inflammation via modulating mucosa-associated microbiota composition in lipopolysaccharides-challenged piglets. Have a positive effect on growth performance in addition to decreasing the crypt depth and enhancing functional development in jejunum of suckling piglets.
Chickens	GOS can modify both cecal tonsil gene expression and the cecal microbiome of chicken, and may be useful as a tool for altering the carriage of Salmonella in poultry. In ovo injection of GOS can mitigate the detrimental effect of heat stress on some meat quality traits of broiler chickens.
Cattle	Supplementation with GOS in early life persistently facilitates the microbial colonization of the rumen and promotes growth of preweaning Holstein dairy calves. Calves receiving GOS experienced better growth performance, increased feed intake, enhanced immune function, and nutrient balance.
Companion animals	GOS–Bifidobacterium pseudocatenulatum combination has some positive effects on the intestinal microbiota in cats. In dogs, GOS supplementation enhances carbohydrate fermentation. Continuous intake of GOS may contribute to canine health.
Aquaculture species	GOS increase Caspian roach fry resistance to salinity stress, improve growth performance, and modulate the intestinal microbiota by increasing lactic acid bacteria (LAB) levels.

Development Workflow of Feed Prebiotics GOS

Why Choose Us?

Our comprehensive technology platform provides a one-stop solution for GOS animal prebiotics development, focusing on feasibility and efficiency.

We possess a range of in vivo and in vitro research models for farm animals and companion animals, enabling comprehensive evaluations of GOS effects in different animal species.

Our advanced omics research platform provides valuable information on microbiome, metabolome, proteome, transcriptome, etc., assisting in the development of GOS prebiotics in animal feed.

BioVenic's research team boasts extensive experience in animal nutrition and prebiotics development, allowing us to quickly understand your needs and provide effective solutions. If you require assistance with production, characterization, in vivo and in vitro studies, finished product development, or validation of GOS as a prebiotic for animal feed, please don't hesitate to contact us. Share your research plan, and we will promptly find a suitable solution for you.