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Custom Genome-wide Libraries in Precision Animal Breeding
Genome-wide gRNA library is a high-throughput gene screening method established based on gene editing technology. It involves functional screening, enrichment, and deep sequencing analysis to identify genes associated with a specific phenotype. BioVenic customizes genome-wide gRNA libraries for precision breeding of farmed animals, providing large-scale screening tools for molecular breeding and promoting the feasibility of efficient and multi-dimensional screening. Our services range from high-throughput sgRNA library construction to virus packaging, cell transfection, high-throughput sequencing, and data analysis, catering to various scientific research needs.
Comparison of Genome-wide Libraries
According to specific research needs, we offer customize services for genome-wide libraries.
| Libraries | Application | Mechanism | Features |
|---|---|---|---|
| Gene-KO Library |
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Non-homologous end joining (NHEJ) repairs DNA double-strand breaks and introduces frameshift mutations in order to achieve knockout. |
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| Gene Activationa Library |
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Dead nuclease is co-expressed with transcriptional activators to effectively increase the expression level of the entire gene. |
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| Gene Interferencei Library |
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Dead nuclease, a transcriptional repressor, was co-transfected into cells with gRNA targeting the upstream regulatory region of the gene in order to achieve gene knockdown. |
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Service Details of Custom Genome-wide Libraries
- Library Design and Production
BioVenic adopts an advanced high-throughput gRNA automated design system to initially design all potential gRNAs and subsequent gRNA screening using a combination of chip-based high-throughput quantitative synthesis and PCR amplification. Our services include assembly of the sgRNA pool into the knockout tool plasmid vector. We perform NGS detection on the vector library, and ultimately accomplishe the construction of the overall library. We customize and construct a high-quality and high-fitness whole-genome library for you.
BioVenic offers a systematic series of services for the preparation and delivery of viral vectors for animal genome editing. We can provide a comprehensive range of customized recombinant viruses, including adenoviruses, adeno-associated viruses, and lentiviruses. BioVenic utilizes a third-generation packaging system and refines it with the optimal particle ratio. For the construction of the entire genome of farmed animals and other genome editing operations, it has high levels of safety, titer, and quality.
- Functional Verification of Screened Genetic Samples
BioVenic offers analysis and verification services for screening data from whole-genome libraries. Using gene editing technology, a single gene knockout project is conducted on the candidate targets obtained through screening to verify the gene function and minimize the interference of false positive results on the overall research progress.
Why Choose Us?
Focus on the construction of genome-wide libraries of farmed animals to fill the market gap.
High editing efficiency library reduces the difficulty of screening and overall research costs.
Strict QC of delivery results through accuracy, coverage and uniformity.
Professional data analysis and result verification are carried out for the screening results.
BioVenic offers analysis and verification services for screening data from whole-genome libraries. Using gene editing technology, a single gene knockout project is conducted on the candidate targets obtained through screening to verify the gene function and minimize the interference of false positive results on the overall research progress.
Reference
- Sanson, Kendall R., et al. "Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities." Nature communications 9.1 (2018): 5416.