Custom CRISPR gRNA Libraries in Precision Animal Breeding

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Custom CRISPR gRNA Libraries in Precision Animal Breeding

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Due to the widespread existence of PAM sites, the establishment of gRNA libraries based on CRISPR technology provides new impetus for screening important gene functions, studying livestock disease mechanisms, and exploring metabolic pathways. BioVenic offers a genome-wide CRISPR gene knockout gRNA library and a transcription activation gRNA library specifically designed for farmed animals. These libraries enable high-throughput and rapid screening of functional genes throughout the entire genome. At the same time, pathway-focused animal gRNA libraries for specific metabolic pathways can also be customized according to the specific project needs.

Services of gRNA Libraries

Custom gRNA Library Design

BioVenic designs the gRNA for a single gene based on specific animal gene information. Based on years of experience in CRISPR projects and a mature gene synthesis platform, our library customization service involves designing and screening multiple gRNAs for each gene. We retain different gRNAs to maximize the success of the editing operation.

gRNA Vector Construction and Library Amplification

For gRNA library construction, BioVenic offers a series of customized services, including vector construction and synthesis, amplification of vector mixed libraries, extraction and purification of plasmid vectors, and more. These services ensure reliable delivery results for our clients.

Virus Packaging

BioVenic offers customization and packaging services for various recombinant viruses, such as adenovirus, adeno-associated virus, and lentivirus. These services are specifically designed for gRNA libraries that target farmed animals. The company's precise control of the ratio allows each virus to carry a single gRNA vector.

gRNA Library Screening and Validation

The target cells are enriched through various screening methods. We offer PCR and NGS sequencing to detect and analyze gRNA libraries, as well as to validate candidate genes, providing customers with high-quality and dependable results.

Fig.1 CRISPR gRNA Library Customization Service Procedure (BioVenic Authorized)

Applications of CRISPR gRNA Libraries

Libraries	Applications
CRISPR-KO gRNA Library	Rapidly generate loss-of-function mutants and screen for desired phenotypes.
Transcription Activation gRNA Library	High-throughput, rapid gain-of-function screening.
Pathway-focused gRNA Library	Screening of pathway or disease-associated genes.

Why Choose Us?

Customized gRNA libraries suitable for genome screening of farmed animals.

Customized gRNA libraries suitable for genome screening of farmed animals.

Various optional delivery methods.

Various optional delivery methods.

Perform NGS sequencing validation to ensure gRNA library quality.

Perform NGS sequencing validation to ensure gRNA library quality.

BioVenic utilizes the precise and powerful features of the CRISPR gene editing tool to develop gRNA library construction services that are suitable for precision animal breeding. Discover key targets after functional screening and enrichment, followed by PCR amplification and deep sequencing analysis, by customizing our genome-wide mutation library or library related to a specific function, constructed using CRISPR technology. If you are interested in any services for gRNA library customization, please feel free to contact us.