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Phage DNA Veterinary Vaccine Development
In addition to phage display veterinary vaccines, phage-based veterinary vaccines also include phage DNA veterinary vaccines. Phages are viruses that infect bacteria and have metabolic inactivity, requiring host bacteria to grow and replicate. As a promising nanomaterial in biotechnology, phage vectors offer numerous advantages over viral and non-viral DNA delivery systems, including enhanced safety and cost-effectiveness. BioVenic provides corresponding services for phage DNA veterinary vaccine development, helping you harness phages as carriers for DNA veterinary vaccines, thereby improving delivery efficiency.
Our Services
Phage Design
In the development of phage DNA veterinary vaccines, the gene encoding the vaccine antigen is cloned into the phage genome under the control of the eukaryotic expression cassette, and the vaccine gene is packaged into the phage particles. BioVenic provides all the services required for the steps in the phage design process to assist you in the development of phage DNA veterinary vaccines.
Gene Synthesis
Construction of Eukaryotic Expression Cassette
Gene Cloning
Fig.1 Schematic diagram of the development of phage DNA vaccines and phage display vaccines 1
Phage Preparation
BioVenic provides comprehensive phage preparation services, which include the following services after cloning the target pathogen antigen gene into the phage genome.
| Phage Cultivation | Phages rely on host bacteria for replication, and BioVenic typically utilizes Escherichia coli for phage amplification.We provide high-quality culture media for bacteria growth to support the development of veterinary vaccines. BioVenic offers custom media formulation services to tailor the media according to your requirements, ensuring optimal culture performance and vaccine products. |
| Phage Extraction and Purification | BioVenic offers various methods to release the phage from the infected bacteria. Typically, we use agitation or employ hypertonic solutions for osmotic shock to facilitate the release. We have established several purification technique platforms for removing residual bacterial cells and other impurities, ensuring that you obtain a pure phage solution, such as centrifugation, filtration, high-speed centrifugation, or column chromatography are employed to separate and purify the phage particles. |
| Phage Verification | BioVenic provides endotoxin removal services and, after completing the removal process, tests for any residual endotoxins. Additionally, purified phages are subjected to negative staining examination using electron microscopy to ensure they meet your requirements. |
Phage DNA Veterinary Vaccine Development Services
Phage cannot replicate in eukaryotic cells, ensuring high safety and no adverse effects on animal health. Due to their adjuvant properties, the introduction of phage DNA vaccines has enhanced the immunogenicity of naked DNA vaccines. BioVenic offers comprehensive in vitro and in vivo testing services to assess the safety, efficacy, and immunogenicity of your developed phage DNA veterinary vaccines. These tests ensure that the vaccines are effective without causing adverse reactions in animals.
BioVenic places a strong emphasis on quality control during the development of veterinary vaccines. We conduct rigorous stability tests to guarantee that your vaccine product maintains high quality under various transportation and storage conditions.
Why Choose Us?
Full-process phage DNA veterinary vaccine development services are offered.
Rigorous and scientific validation services ensure that phage vector construction and preparation meet your research and development requirements.
The assessments of safety and efficacy guarantee the success of your veterinary vaccine products.
DNA vaccine administration involves the integration of target antigen gene sequence into plasmid DNA, allowing it to be expressed within host cells, providing protection against various pathogens. Compared to traditional veterinary vaccines, DNA veterinary vaccines are more cost-effective and have fewer adverse effects. With extensive experience in developing phage-based veterinary vaccines, BioVenic is your ideal partner developing phage display vaccines or phage DNA vaccines for livestock, poultry, companion animals, aquaculture species and more. Please contact us and our professional team and advanced equipment will guide you toward success in veterinary vaccine development!
References
- Jafari, Narjes, and Saeid Abediankenari. "Phage particles as vaccine delivery vehicles: concepts, applications and prospects." Asian Pac. J. Cancer Prev 16 (2015): 8019-8029.
- Xu, Hai, et al. "Engineering T7 bacteriophage as a potential DNA vaccine targeting delivery vector." Virology journal 15.1 (2018): 1-8.