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Veterinary Loop-Mediated Isothermal Amplification (LAMP) Kit Development

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Loop-mediated isothermal amplification (LAMP) is a new nucleic acid amplification technology that is "simple, fast, accurate, and low-cost". Recently, LAMP has been increasingly applied to veterinary diagnosis, bringing about refreshing changes. BioVenic is a leading company specializing in advanced molecular biology solutions for the veterinary industry. Our expertise in veterinary LAMP/RT-LAMP kit development provides excellent service to our customers.

What Are LAMP and RT-LAMP?

LAMP

The loop-mediated isothermal amplification (LAMP) method allows rapid and specific amplification of DNA under isothermal conditions. Unlike traditional PCR, LAMP does not require a thermal cycler, making it a cost-effective and time-saving alternative. The LAMP reaction involves the use of multiple primers and a DNA polymerase with strand-displacement activity to achieve exponential amplification of the target DNA sequence.

RT-LAMP

Reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) combines the principles of LAMP with reverse transcription to amplify RNA targets. This innovative approach has revolutionized RNA detection and analysis, particularly in the field of veterinary diagnostics.

Schematic diagram of the principle of LAMP Fig. 1 Schematic diagram of the principle of LAMP.1

What We Offer?

BioVenic's veterinary LAMP/RT-LAMP kit development services cover the entire process from initial design to custom kit production. We provide comprehensive services to ensure that help customers develop LAMP kits of high quality and performance. Our advantageous services include the following items.

Kit Development and Optimization

By considering factors such as target pathogens or genetic markers, sample types, and specific detection requirements, we provide primer design services and select components to help customers develop LAMP/RT-LAMP kits for animal diagnostic research.

Customization and Tailored Solutions

We understand that each customer has unique requirements. Our team works closely with our customers to customize the LAMP/RT-LAMP kits according to their specific needs.

Quality Control and Validation

BioVenic maintains strict quality control measures to ensure that our veterinary LAMP/RT-LAMP kits meet the highest standards.

LAMP/RT-LAMP Kit Production

Our contract manufacturing service enables the production of kit for research use, including the preparation of master mixes, primers, enzymes, and other necessary components.

Common Viruses Detected by LAMP/RT-LAMP in Veterinary Diagnostics

LAMP and RT-LAMP can detect a wide variety of pathogens, including viruses, bacteria, parasites, and protozoa among other pathogens. Viruses and bacteria are the focus of current research. We have rich experience in developing virus/bacteria LAMP/RT-LAMP detection kits for customers. The table below shows some of our service capabilities.

Veterinary LAMP/RT-LAMP Kit Development Service Process

At BioVenic, we follow a rigorous development process to ensure the quality and reliability of our preclinical veterinary NGS panels or kits. This process involves several key stages as follows.

Design Primers

We help customers design and synthesize LAMP/RT-LAMP primers for target genes, including forward inner primer, forward outer primer, backward inner primer, and backward outer primer.

Template Preparation

We help customers prepare template DNA or RNA, which can be purified nucleic acid or nucleic acid extracted directly from a sample.

Results Visualization

We analyze results for customers through real-time quantitative detection or end-point detection methods, such as fluorescent dyes, colorimetric methods, gel electrophoresis, latex agglutination, etc.

Veterinary LAMP/RT-LAMP Kit Development Service Process

Reaction System Setup

We provide LAMP/RT-LAMP master mix preparation service. The premix can be prepared by using commercial products directly, or by preparing a reaction system containing Bst DNA polymerase, reverse transcriptase, buffer, dNTPs, etc.

Reaction Condition Setting

We set the appropriate reaction conditions for our customers. The LAMP/RT-LAMP reaction is carried out under constant temperature conditions (usually 60-65℃), and the reaction time depends on the target copy number and the performance of the premix solution, generally 5-60 minutes.

Applications of LAMP and RT-LAMP in Veterinary Diagnostics

Applications of LAMP and RT-LAMP in Veterinary Diagnostics

LAMP and RT-LAMP techniques offer rapid, sensitive, and precise identification of diverse veterinary pathogens, including viruses, bacteria, and more.

Applications of LAMP and RT-LAMP in Veterinary Diagnostics

Ease of operation and visualized outcomes make LAMP and RT-LAMP suitable for epidemiological studies and investigations of various animal pathogenic microorganisms.

Applications of LAMP and RT-LAMP in Veterinary Diagnostics

LAMP and RT-LAMP are often used for the diagnosis and monitoring of infectious diseases in companion animals, such as canine distemper virus.

BioVenic stands as a reputable company within the veterinary research, crafting cutting-edge solutions within the realm of molecular biology. We hold steadfast confidence in delivering top-tier veterinary LAMP/RT-LAMP kit development services of the utmost quality. Should you have questions or require further information, please do not hesitate to directly contact us. Your inquiries are of paramount importance to us.

Reference

  1. Park, Jee-Woong. "Principles and applications of loop-mediated isothermal amplification to point-of-care tests." Biosensors 12.10 (2022): 857.
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