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Custom Animal Cell 3D Culture Development
3D cell culture provides an unparalleled platform for animal cell research, enabling the reconstruction of the spatial arrangement of animal cells in vitro. These systems have gained increasing attention due to their clear advantages over 2D cell culture systems, offering more physiologically relevant information and predictive data. To cater to the diverse technical requirements of researchers, BioVenic has established an advanced platform for developing animal cell 3D culture systems. We support the development of scaffold systems and serum-free culture systems, and serve as a reliable scientific research partner for the design and execution of research programs in emerging fields such as artificial flesh, organoids, animal cell therapy research, and veterinary drug development.
Background
The maturity and standardization of animal cell culture technology enables researchers to replicate the in vivo environment in vitro to maintain cell structure and function and to conduct early animal drug development or basic life science research. Traditional cell culture involves the cultivation of a monolayer of cells in a suitable culture vessel, also known as 2D cell culture. However, researchers have found that this experimental method can lead to changes in cell morphology, abnormal division, and partial loss of differentiation activity during the cell culture process, resulting in significant differences in cell morphology, function, and stress response compared to the in vivo environment. The cell model thus created is far removed from the actual conditions in the body. Recognizing the need to mimic as closely as possible the state of cells in the body during in vitro culture, the 3D culture system was developed to address many of the shortcomings of the monolayer cell culture process.
Table. 1 2D Cell Culture VS 3D Cell Culture
| 2D Cell Culture | 3D Cell Culture |
|---|---|
| Cells adhere flatly to the surface of the culture dish. | Simulates the in vivo cell microenvironment. |
| Cannot accurately depict the true morphology and structure of the organism. | Simulates cell-cell interactions. |
| Lacks a microenvironment conducive to cell survival. | Simulates the biochemical and physiological responses of cells. |
| Unrestricted access to essential compounds. | Exhibits heterogeneous cell polarity and phenotypes. |
| Lack of interaction between cells and the environment. | Overcomes the ethical concerns of using animal models. |
Fig. 1 Schematic Diagram of 3D and 2D Animal Cell Culture Status
Animal Cell 3D Culture Services
BioVenic offers tailored services for researchers looking for solutions for in vitro three-dimensional culture of animal cells, which are mainly categorized into scaffold-based 3D cell culture and scaffold-free 3D cell culture. The former utilizes natural or artificial microporous materials with excellent biocompatibility to construct a scaffold with gaps for cell growth. Cells depend on it as a support and thrive in three-dimensional space. The latter employs the principle of cell self-aggregation to facilitate the formation of cell microspheres by establishing a growth environment conducive to cell aggregation.
Scaffold-based 3D Cell Culture
BioVenic has mastered the development of traditional and mature scaffold-based culture systems. Scaffold materials include, but are not limited to, agarose, collagen, fibronectin, gelatin, laminin, etc. We simulate the in vitro culture of cells from different tissues by adjusting key parameters of the materials, such as porosity, fiber, permeability, and mechanical stability. We reproduce the interactions between cells and the interaction between cells and extracellular matrix in the in vivo environment. Simultaneously, it allows cells to aggregate, proliferate, and migrate on the scaffold to maintain cell viability.
Scaffold-free 3D Cell Culture
BioVenic supports the development and implementation of scaffold-free 3D culture solutions for animal cells. Our technicians use cell culture vessels treated with cell-repellent materials or special systems such as hanging drops and microfluidics to carry out cell culture. This prevents cells from attaching to the surface of the vessel and forces them to aggregate into cell microspheres. The 3D cell model obtained by this method has a complete spherical structure, can spontaneously form an extracellular matrix, and effectively simulates the oxygen and nutrient gradients present in the in vivo environment.
Applications of Animal 3D Cell Culture Technology
- Veterinary Drug Development and Animal Disease Models
Although the use of 2D cell models helps to reveal many physiological and pathological processes in animals, they cannot simulate the complex cellular microenvironment in the animal body, resulting in inconsistencies in in vivo and in vitro pharmacological or toxicological effects. 3D cell models are more insightful and predictive in the veterinary drug evaluation process. They can also avoid the use of animal models and reproduce some key cell types and structural characteristics of the represented organs.
- Using Stem Cells to Build Organoids
2D cell culture cannot accurately replicate the in vivo microenvironment of stem cells, which reduces their ability to proliferate and differentiate. Stem cells obtained through 3D cell culture can exhibit similar cell morphology, stress response, and gene expression patterns as in vivo. In addition, cell activity and survival rates are significantly enhanced. The technical approach for developing veterinary organoids using cultured stem cells for 3D cell culture has also advanced, resulting in a three-dimensional structure resembling real organs and expressing similar markers.
- Cell Cultured Meat Development and Production
Three-dimensional cell culture in vitro is a crucial technology for the introduction of cell cultured meat into the market. Hydrogel serves as a scaffold material to offer 3D support for cell culture, enabling artificial meat to have fat, muscle, blood vessels, and a taste that closely resembles real meat.
Why Choose Us?
Ensure Cell Viability
Our technical team efficiently separates primary cells, minimizing the loss of cell viability, and ensuring cell proliferation and differentiation during the 3D culture process.
Make Good Use of Cytokines
BioVenic is committed to using safe, effective, and consistently high-quality cytokines to promote cell growth, repair, and differentiation during 3D culture.
Rich Project Experience
We have accumulated many years of project experience in separating animal stem cells and primary cells, as well as designing and developing in vitro culture systems to ensure the program's reliability.
BioVenic has developed a tailored technical service platform for in vitro cultivation of diverse animal tissue cells, with a focus on advancing 3D culture systems. We offer customized solutions for researchers seeking to establish animal cell 3D culture initiatives. Our wide range of services ensures a close alignment with the specific requirements and objectives of each customer's project. If you are interested in creating animal cell culture programs, please contact us for comprehensive information and quotations.