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Veterinary ELISA Development
Veterinary ELISA development is an important aspect of animal health research and diagnostics. This technique is used to qualitatively or quantitatively detect the presence of specific antigens, antibodies, or other molecules in animal samples such as blood or urine to diagnose disease or monitor animal health. In veterinary ELISA assay applications, BioVenic is a leading company. Our expert team can work with you to design and develop a custom ELISA assay that meets your specific needs.
ELISA Kit Formats
There are many forms of ELISA kits, direct ELISA, indirect ELISA, competition ELISA and sandwich ELISA are the main and most common four. However, our R&D capabilities are not limited to these four types. ELISA kits in the form of plasmonic ELISA, bead-based ELISA, paper-based ELISA, and microfluidic ELISA are also used in many applications.
Fig. 1 Four common formats of ELISA kits.1
Veterinary ELISA Development Services
BioVenic offers a range of services for veterinary ELISA development to ensure the accuracy and reliability of our assays. Our team of experienced scientists uses advanced technology and equipment to ensure our assays are of the highest quality and apply to a wide variety of samples. Our services are as follows.
- Identify suitable capture and detection antibodies for target molecules.
- Custom veterinary ELISA antibody-antigen Development.
- Optimize assay conditions such as antigen concentration, buffer composition, and incubation time.
- Validation of assay performance, including sensitivity, specificity, and reproducibility.
- Cross-reactivity testing with related molecules to ensure that the assay is specific for the molecule of interest.
Veterinary ELISA Development Process
Consulting
Our team of experts will work with clients to understand their specific research or diagnostic needs and develop a custom assay plan.
Assay Optimization
Our service optimizes assay conditions such as antigen concentration, buffer composition, and incubation time to ensure the highest possible sensitivity and specificity.
Production
We provide our customers with small-scale laboratory production or large-scale manufacturing.
Antibody Selection
We provide antibody development services using our extensive antibody database to select the appropriate capture and detection antibodies for your target molecules.
Validation
Our service validates assay performance by testing with known positive and negative samples and measuring sensitivity, specificity, and reproducibility.
Why Choose Us?
BioVenic has several advantages that make us the first choice for veterinary ELISA development.
Expertise
Our team of scientists has extensive experience in developing and optimizing ELISA assays for veterinary diagnostics.
Customization
Our services are highly customizable to meet the specific needs and requirements of our clients.
Multiple animal species
Our development services target companion animals such as dogs and cats, as well as various livestock animals such as pigs, cows, and chickens.
Broad target
Our target range includes veterinary viruses, bacteria, parasites, animal pregnancy, cardiovascular disease, and cancer markers, among others.
BioVenic has extensive experience in developing ELISA kits. The veterinary ELISA development services offered by BioVenic help you find appropriate solution for animal disease diagnostic research. Contact us today to learn more about how we can help you develop custom veterinary ELISA kits.
Reference
- Gao, Yuan, et al. "Metal and metal oxide nanoparticles to enhance the performance of enzyme-linked immunosorbent assay (ELISA)." ACS Applied Nano Materials 3.1 (2019): 1-21.
Case Studies
BioVenic: Custom Veterinary ELISA Development
Application: Veterinary Diagnostics
| Parameter | BioVenic |
|---|---|
| Specificity/Target | Alpha 1-Acid Glycoprotein |
| Detection Range | 6.17 ng/mL - 1500 ng/mL |
| Sensitivity | 1.706 ng/mL |
| Reactivity | Bovine/Cow |
| Sample Type | Plasma, serum |
| Storage Conditions | 2-8℃ |
BioVenic: Custom Veterinary ELISA Development
Application: Veterinary Diagnostics
| Parameter | BioVenic |
|---|---|
| Specificity/Target | Haptoglobin |
| Detection Range | 6.25 ng/mL - 400 ng/mL |
| Sensitivity | 4.261 ng/mL |
| Reactivity | Feline/Cat |
| Sample Type | Plasma, serum |
| Storage Conditions | 2-8℃ |
ELISA Development FAQs
Companion animals such as canines, felines, rabbits, etc. Livestock animals include equine, bovine, caprine, porcine, sheep, poultry, ovine, and goat.
Customers do not need to provide antigens and antibodies, and you can entrust us to purchase or prepare antibodies or antibody pairs and high-purity antigens for you.
- Ensure that the environment and operators of each inspection are the same to reduce random errors.
- Ensure that reagents such as antibodies, enzymes, and substrates are stored at the temperature specified in the instructions to ensure the quality of the reagents.
- Avoid contamination of samples or reagents during the test.
- Operate according to the instructions and avoid the incubation temperature being too high and the time is too long.
ELISA is a highly sensitive assay. The ELISA kit we developed for you has been strictly verified and has a high accuracy rate.
There are two major types of ELISA, based on the substrate and corresponding products, colorimetric and chemiluminescent. A colorimetric method uses the OD value to determine the amount of the analyte, while a chemiluminescence method uses a photometer and a standard curve to determine the amount.