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Veterinary Fluorescence in Situ Hybridization (FISH) Assay Development

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In the field of veterinary medicine, fluorescence in situ hybridization (FISH) has attracted increasing attention as a diagnostic tool that is good at revealing diseases such as pathogens and tumor abnormalities in animals. BioVenic has been a leader in veterinary diagnostics for years and has become a preeminent CRO company, providing clients with cutting-edge veterinary FISH assay development services and assist veterinary researchers in their animal disease diagnosis studies.

Features of FISH

FISH is a technique that utilizes fluorescently labeled probes to target specific DNA or RNA sequences in a sample. The probe hybridizes with the complementary target sequence in the sample, and the fluorescent signal emitted by the probe allows for visualization and identification of the target sequence under a microscope. FISH's unique features make it a valuable diagnostic tool in veterinary medicine.

FISH is highly specific, allowing for the detection of target sequences with high accuracy

FISH is highly specific, allowing for the detection of target sequences with high accuracy.

FISH is a rapid technique that can produce results in a matter of hours

FISH is a rapid technique that can produce results in a matter of hours.

FISH can be performed on a variety of sample types, including tissue sections, cytological specimens, and even whole blood

FISH can be performed on a variety of sample types, including tissue sections, cytological specimens, and even whole blood.

Schematic diagram of how the FISH technique works.Fig. 1 Schematic diagram of how the FISH technique works.1

Veterinary FISH Assay Development Services

BioVenic provides comprehensive veterinary FISH assay development services, fully meeting customer needs in terms of sample type, probe type, FISH format, kit type, target, etc. We show some of our capabilities below for understanding your research needs.

FISH exhibits prowess in the identification of both pathogens and genetic anomalies within the animal domain. Our expertise extends to the formulation of FISH kits designed to pinpoint an extensive spectrum of bacteria, viruses, and parasites, as well as chromosomal rearrangements and gene amplifications. Provided below is a table that enumerates a selection of the targets we proficiently address.

Veterinary FISH Development Process

Step 1. Design and Prepare Fluorescently Labeled Probes

Our scientists help to select the appropriate probe length, sequence, marker, and labeling method, and prepare probes with high specificity, sensitivity, and stability, according to the sequence and characteristics of the target nucleic acid.

Step 2. Optimize and Validate Hybridization Conditions

Our scientists select the appropriate hybridization buffer, temperature, time, and other parameters according to the type of probe and sample we are testing, to ensure hybridization efficiency and signal-to-noise ratio. Validate probe sensitivity and specificity using known positive and negative samples.

Step 3. Provide Related Reagents and Accessories

We provide customers with reagents such as fixative, blocking solutions, washing solutions, staining solutions, and anti-fading agents, as well as accessories such as cover glass and parafilm that are suitable for the application range of the kit.

Step 4. Provide Operating Instructions

We help customers write detailed, clear, and standardized operation manuals, describing the composition, storage conditions, precautions, procedures, and results of the kits.

Step 5. Developed FISH Kits Contract Manufacturing

We provide subsequent contract veterinary FISH IVD kit manufacturing services according to customer needs.

Veterinary FISH development flow chart. (BioVenic Original)

Applications

  • FISH marks and localizes tumor DNA for diagnosis of canine or feline tumors, tumor typing, etc.
  • FISH is used to detect veterinary pathogenic microorganisms such as bacteria, viruses, protozoan, and fungi, and facilitate the diagnosis of animal diseases.
  • FISH is used to monitor animal health by directly observing chromosome morphology and chromatin within the nucleus.

Why Choose Us?

Diverse animal expertise

Diverse animal expertise

Our expert team has developed FISH kits for a variety of animal species to meet the different needs of customers.

Holistic veterinary FISH solutions

Holistic veterinary FISH solutions

Our repertoire encompasses an all-encompassing array of veterinary FISH development services. From the inception of custom probe design to meticulous probe synthesis, optimization, and rigorous testing.

Meticulous attention to detail

Meticulous attention to detail

Every facet of our approach is infused with a dedicated focus on the minutest details. This encompassing dedication extends to our probe design, ensuring the formulation of FISH detection solutions that are both precise and sensitive.

Recognized as the leading bio company specializing in veterinary FISH assay development services, BioVenic takes the forefront in delivering unmatched solutions. Don't hesitate to contact us to delve into the intricacies of our services and uncover how our expertise can significantly enhance your veterinary diagnostic studies.

Reference

  1. Amann, Rudolf, et al. "Single-cell identification in microbial communities by improved fluorescence in situ hybridization techniques." Nature Reviews Microbiology 6.5 (2008): 339-348.
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