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Veterinary Lateral Flow Assay Development
The rapid immunodiagnostic test known as the lateral flow assay (LFA) employs antibodies to swiftly identify the presence of a targeted analyte within a sample. With the ongoing progression of the veterinary sector, the demand for precise and effective diagnostic instruments becomes increasingly pivotal. LFA analysis has emerged as a prominent solution in response to this need. At BioVenic, our expertise lies in the meticulous creation and production of LFA test strips designed for veterinary implementations. These strips deliver dependable and precise outcomes, catering to a wide array of diagnostic investigations.
How Does Lateral Flow Test Work?
The architecture of a LFA comprises various integral elements, including a sample pad, a conjugate pad, a nitrocellulose membrane, and an absorbent pad. Its workflow begins with introducing the sample onto the sample pad and across the conjugate pad. At this juncture, antibodies tagged with a detection label engage with the specific target analyte. Subsequently, the sample advances to the nitrocellulose membrane, where immobilized antibodies capture the analyte-detection label complex. Ultimately, the sample concludes its journey at the absorbent pad, responsible for absorbing any unbound constituents.
Fig. 1 Construction diagram of lateral flow test.1
Veterinary LFA Development Services
At BioVenic, our team of experienced scientists and technicians work closely with our customers. We offer comprehensive veterinary LFA development services covering antigen selection, antibody preparation, antibody conjugation, membrane selection, and optimization, LFA assembly, validation and optimization, manufacturing, and quality control.
There are a variety of factors to consider when developing a lateral flow test, and we can do it all for you. Some popular service menus are listed in the table below.
| Consider Factors | Popular Test Menu |
|---|---|
| Types of lateral flow tests | Sandwich assays, competitive assays |
| Sample types | Milk, whole blood, serum, saliva, urine, tissue, food, feed, water, etc. |
| Label types | Combine gold, colored latex nanoparticles, magnetic or colored polystyrene beads, etc. |
| Test strips and dipsticks | Isotyping test strips or dipsticks, anti-cytokine test strips, control rapid strips, etc. |
Veterinary Lateral Flow Assay Development Process
In the process of advancing veterinary LFA development, we incorporate a diverse array of analytical methodologies to meticulously assess the efficacy of FLA, including ELISA, SPR, and fluorescence microscopy.
Customer Consultation
Communicate with customers in detail to determine target analytes and understand specific diagnostic requirements.
Antibody and Probe Selection
Our service selects a suitable antibody or probe with strong specificity based on the determined analyte.
Optimize Labeling Conditions
Our service involves carefully balancing factors such as antibody concentration, conjugation chemistry, and reaction time.
Membrane Selection and Optimization
Our service will tests different membrane materials and pore sizes for optimal performance.
Assembly of Lateral Flow Assay
Our service coats nitrocellulose membranes with capture antibodies or probes and immobilizes detection antibodies or probes on conjugate pads.
Validation and Optimization
We provide validation and optimization services based on the use of known positive and negative samples to assess the sensitivity and specificity of the LFA assay.
Why Choose Us?
Our development services are dedicated to improving the sensitivity and specificity of preclinical lateral flow tests.
In our R&D services, we specialize in integrating multiple probes in tests and enabling multiplexing.
We fully realize the digital connection and green manufacturing and develop high-accuracy and advanced pre-clinical lateral flow tests for you.
When it comes to veterinary lateral flow assay development, BioVenic stands as the optimal collaborator. Personalized services, meticulous protocols, stringent quality control, and unmatched custom service position us as the ideal choice for you to explore pertinent research. For any queries or information, please don't hesitate to contact us directly.
Reference
- Budd, Jobie, et al. "Lateral flow test engineering and lessons learned from COVID-19." Nature Reviews Bioengineering 1.1 (2023): 13-31.