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Veterinary Western Blot Assay Development
BioVenic is a leading provider of veterinary Western blot assay development services. We have been in the industry for years, and our team of experts has extensive experience in developing Western blot assays for the detection of various proteins in veterinary samples.
What Is Western Blot?
Western blotting is a method to detect and measure specific proteins in a sample. It involves the following steps.
- Breaking down the sample into proteins and separating them by size using a gel.
- Transferring the proteins from the gel to a membrane and blocking extra spaces.
- Adding antibodies that bind to the target protein and make it visible.
- Capturing the signal from the antibodies using color, light, or fluorescence.
- Analyzing the signal to identify and quantify the protein.
Fig. 1 Detection flowchart of Western blotting.1
Veterinary Western Blot Assay Development Services
BioVenic offers comprehensive veterinary western blot development services based on client needs. We offer you a vast selection of animal species, pathogens, disease biomarkers, and assay accessories. The table below lists some of our service capabilities.
| Parameters | Popular Projects |
|---|---|
| Animal species | Horses, mules, feline, canine, rabbit, bovine, porcine, sheep, poultry, ovine, goat, and other livestock and companion animals. |
| Pathogens | Capripox virus, equine infectious anemia virus, Glanders, Trichinella specie, Leishmania, Mycobacterium avium complex, Mycoplasma agassizii, and other bacterial, viral, and parasitic pathogens. |
| Biomarkers | Viral infection markers: VP2 protein, NP protein, S protein, etc. |
| Parasite infection biomarkers: AFP, CEA, CA125, CA19-9, etc. | |
| Cyclins: cyclin D1, cyclin E, p21, p27, etc. | |
| DNA damage repair proteins: p53, BRCA1, BRCA2, PARP, etc. | |
| Stem cell differentiation-related proteins: Oct4, Sox2, Nanog, Klf4, etc. | |
| Apoptosis-related proteins: caspase 3, caspase 8, caspase 9, Bcl-2, etc. | |
| Accessories | Chromogenic substrate, lysis solution, blocking solution, gel, transfer membrane, etc. |
Veterinary Western Blot Assay Development Process
The veterinary Western blot assay development process is critical for the detection and diagnosis of various animal diseases. To ensure the reliability and sensitivity of the assay, each step must be carefully optimized and validated.
Our team of experts consults with the customer to understand their requirements and needs for the veterinary western blot assay development process.
Synthesis, purification, and/or expression of the antigen(s) of interest, as determined by the client's needs.
Sample preparation is an important step in performing Western blotting. Our platform enables protein extraction and purification.
It is necessary to select the gel percentage, specific primary and secondary antibodies, nitrocellulose membrane or PVDF membrane, blocking solution, and chromogenic substrate according to the protein to be analyzed, and perform image analysis, data analysis, etc.
Our team will optimize the test conditions, including the selection of appropriate primary and secondary antibodies, antibody concentrations, and incubation times, to ensure maximum sensitivity and specificity of the assay.
We offer fully validate the assays with positive controls, negative controls, secondary antibody controls, blank controls, and loading controls to ensure that it meets the required sensitivity and specificity criteria.
After validation, we would submit a detailed synthesis report including a detailed description of the methods used, the results obtained, and any relevant conclusions or recommendations.
Our Service Flow Chart
Why Choose Us?
We offer veterinary western blot assay development services for a range of pathogens.
Provides the preparation of recombinant antigens to improve the specificity and reproducibility of detection.
Use a variety of detection methods, such as chemiluminescence or fluorescent labeling, to visualize target protein bands on the membrane.
Strict quality control measures, using 5 kinds of control samples to ensure the accuracy and reliability of the test results.
BioVenic understands the importance of accurate and reliable preclinical veterinary diagnostic assays. We help customers develop WB-based diagnostic research tools that meet their requirements for animal species, pathogens, biomarkers, accessories, etc. Contact us today to learn more about our veterinary WB assay development services.
References
- Kroon, Cristina, et al. "Blind spots on western blots: Assessment of common problems in western blot figures and methods reporting with recommendations to improve them." PLoS biology 20.9 (2022): e3001783.
- Trevisan, Daliah Alves Coelho, et al. "Diagnostic methods to cutaneous leishmaniasis detection in domestic dogs and cats." Anais Brasileiros de Dermatologia 90 (2015): 868-872.