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Veterinary Agar Gel Immunodiffusion (AGID) Assay Development

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BioVenic is a leading veterinary diagnostics company dedicated to pioneering animal health advancements. An exemplary service we provide is the development of veterinary agar gel immunodiffusion (AGID) assays. This technology has broad significance in identifying antibodies against infectious agents in animals.

How Does the AGID Assay Work?

The AGID assay is a highly specific and sensitive technique for detecting antibodies against infectious agents in animals. It is a simple and easy-to-perform test, which can be carried out in most laboratory settings. In the AGID assay, serum samples and antigen preparations are placed in separate wells in an agar gel plate, from which they diffuse into the gel. A thin white line precipitates in the gel if the serum contains antibodies that recognize the antigen.

Schematic diagram of the veterinary AGID assay.Fig. 1 Schematic diagram of the veterinary AGID assay.1

Veterinary AGID Assay Development Services

BioVenic offers comprehensive veterinary AGID assay development services, which include the design, optimization, and validation of the assay for specific animal disease diagnostic indications. The AGID assay development process at BioVenic is a multi-step process that involves several key stages.

Step 1. Initial Consultation

We conduct an initial consultation with the client to understand their specific needs and requirements for the AGID assay. This includes defining the target analyte, sample matrix, and desired sensitivity and specificity of the assay.

Step 2. Antigen Preparation

We offer target antigen preparation, purification, and characterization services. The step is crucial to ensure that the antigen is of high quality and free from contaminants, which could interfere with the assay.

We provide polyclonal or monoclonal antibody production services, which is designed against the target antigens. The choice of antibody type depends on the assay's requirements, such as the specificity and sensitivity needed.

Step 4. Test Development

We use the produced antibodies to develop the AGID assay. The process includes optimizing the antigen-antibody ratios, buffer composition, and other critical parameters to achieve the desired sensitivity and specificity.

Step 5. Validation

Once the assay is developed, it undergoes rigorous validation to ensure that it meets the required specifications. This includes determining the limit of detection, limit of quantification, specificity, and reproducibility.

We help optimize the production processing of veterinary AGID assay and provide scale-up manufacturing services.

Common Pathogens to be Tested

The AGID assay can be used to detect antibodies against various pathogens that cause infectious diseases. Popular veterinary diagnostic research programs using AGID assay include but are not limited to,

  • Influenza A virus,
  • Mycobacterium avium subspecies paratuberculosis (MAP),
  • Histoplasma capsulatum,
  • Equine infectious anemia virus.

Our Service Flow Chart

Customer Inquiry

Assay Validation

Assay Design and Optimization

Results Submission

Why Choose Us?

Our team of experienced scientists and technicians possess extensive knowledge and expertise in AGID, ensuring that your project is in good hands

Our team of experienced scientists and technicians possess extensive knowledge and expertise in AGID, ensuring that your project is in good hands

Our AGID assay development services target a wide range of veterinary pathogens and can be tailored to your specific requirements

Our AGID assay development services target a wide range of veterinary pathogens and can be tailored to your specific requirements.

Our AGID assay development process is designed to be efficient, ensuring that we can deliver results in a timely manner

Our AGID assay development process is designed to be efficient, ensuring that we can deliver results in a timely manne.

BioVenic is your ideal partner for AGID assay development. Our team of experts, state-of-the-art facilities, and commitment to quality make us your first choice for preclinical AGID assay development. Contact us today to learn more about our AGID assay development services.

References

  1. Nemoto, Manabu, et al. "Comparison of two agar gel immunodiffusion protocols for diagnosing equine infectious anemia." Journal of Veterinary Medical Science 80.8 (2018): 1245-1247.
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