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Veterinary Immunofluorescence Assay (IFA) Development
Immunofluorescence assay (IFA) holds widespread prominence within the domain of veterinary medicine, facilitating the identification of pathogens, antibodies, and other pertinent biomolecules. This technique harnesses antibodies that are fluorescently tagged, enabling them to selectively attach to designated targets, thereby enabling visualization and quantification. BioVenic specializes in the development of custom veterinary IFA for a wide range of applications.
What Is IFA?
IFA is a laboratory technique that uses fluorescently labeled antibodies as probes to localize and qualitatively analyze a specific target antigen or protein of interest within tissues or cells. This technique can be divided into direct and indirect methods. The direct IFA is to directly react the labeled specific fluorescent antibody with the antigen, and the indirect IFA is to use the unlabeled specific antibody to react with the antigen sample first, and then use the labeled anti-antibody to react with it.
Fig. 1 Schematic diagram of indirect IFA.1
Veterinary IFA Development Services
The development of an IFA assay typically involves multiple steps, including antigen selection, antibody production, and assay optimization. BioVenic has a team of experienced scientists and assists customers to develop immunofluorescence assay.
Our team of experienced scientists works closely with customers to develop customized assays that meet their unique needs. The popular choices are shown in the table.
| Parameters | Details |
|---|---|
| Sample Types | Cells, tissue, serum |
| Fluorescent Dyes | Fluorescein isothiocyanate (FITC), tetraethylrhodamine, tetramethylrhodamine isothiocyanate (TRITC), phycoerythrin (R-RE), etc. |
| Popular Targets |
|
Veterinary IFA Development Process
Consultation
Determine the purpose and design of the experiment, and select the appropriate sample type, antigen target, antibody type, and fluorescent marker.
IFA Development
Our services process samples according to customer needs, including fixation, permeabilization, sealing, etc., and incorporation of specific antibodies to develop targeted diagnostic kits.
IFA Validation
Our service validates the IFA using a series of positive and negative controls to ensure it meets the necessary standards of accuracy and reliability.
Custom Antibody Production
We offer custom antibody production services to generate high-quality antibodies that specifically bind your biomolecules of interest.
IFA Optimization
We provide optimization services for primary and secondary antibodies, including concentrations, incubation times and conditions, and fluorescent dyes, to determine optimal detection conditions.
Applications
- Detect the expression level and subcellular localization of biologically active substances of interest in animal cells or tissues.
- Test for antibodies or antigens in animal serum for infectious diseases, endocrine disorders, tumors, drug testing, immunology, blood group identification, etc.
- Analyze the morphological structure and function of animal cells or tissues, such as cell cycle, apoptosis, proliferation, differentiation, migration, etc.
Why Choose Us?
Detect the expression level and subcellular localization of biologically active substances of interest in animal cells or tissues.
Test for antibodies or antigens in animal serum for infectious diseases, endocrine disorders, tumors, drug testing, immunology, blood group identification, etc.
Analyze the morphological structure and function of animal cells or tissues, such as cell cycle, apoptosis, proliferation, differentiation, migration, etc.
BioVenic's veterinary IFA development services provide a comprehensive solution for developing studies for preclinical veterinary diagnostics. We meet the needs of customers for sample types, fluorescent dyes, and targets of viruses, bacteria, and parasites. Please contact us to learn more details.
Reference
- Ryu, Wang-Shick. "Diagnosis and methods." Molecular virology of human pathogenic viruses (2017): 47.